Nuclear and cytoplasmic genome composition of Solanum bulbocastanum (+) S. tuberosum somatic hybrids. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Nuclear and cytoplasmic genome composition of Solanum bulbocastanum (+) S. tuberosum somatic hybrids. (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1139/G07-024 (literal)

Alternative label

• Iovene M, Savarese S, Cardi T, Frusciante L, Scotti N, Simon P, Carputo D (2007)
  Nuclear and cytoplasmic genome composition of Solanum bulbocastanum (+) S. tuberosum somatic hybrids.
  in Genome (Ott., Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Iovene M, Savarese S, Cardi T, Frusciante L, Scotti N, Simon P, Carputo D (literal)

Pagina inizio

• 443 (literal)

Pagina fine

• 450 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 50 (literal)

Rivista

• Genome (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• (1) CNR-IGV - Institute of Plant Genetics; (2) University of Naples Federico II - Department of Soil, Plant and Environmental and Animal Production Sciences; (3) USDA-Agricultural Research Service - Department of Horticulture (literal)

Titolo

• Nuclear and cytoplasmic genome composition of Solanum bulbocastanum (+) S. tuberosum somatic hybrids. (literal)

Abstract

• Somatic hybrids between the wild incongruent species Solanum bulbocastanum (2)1 = 2x = 24) and S. tuberosum haploids (2n = 2x = 24) have been characterized for their nuclear and cytoplasmic genome composition. Cytologic observations revealed the recovery of 8 (near-)tetraploid and 3 hexaploid somatic hybrids. Multicolor genomic in situ hybridization (GISH) analysis was carried out to study the genomic dosage of the parental species in 5 somatic hybrids with different ploidy. The GISH procedure used was effective in discriminating parental genomes in the hybrids; most chromosomeswere unambiguously colored. Two (near-)tetraploid somatic hybrids showed the expected 2:2 cultivated-to-wild genomic dosage; 2 hexaploids revealed a 4:2 cultivated-to-wild genomic dosage, and I hexaploid had a 2:4 cultivated-towild genomic dosage. Characterization of hybrid cytoplasmic genomes was performed using gene-specific primers that detected polymorphisms between the fusion parents in the intergenic regions. The analysis showed that most of the somatic hvbrids inherited the plastidial and mitochondria) DNA of the cultivated parent. A few hybrids, with a rearranged mitochondrial genome (showing fragments derived from both parents), were also identified. These results confirmed the potential of somatic hybridization in producing new variability for genetic studies and breeding. (literal)

Prodotto di

• Ottenimento di nuovi prodotti per la salute, l'alimentazione e l'industria mediante interventi genetici e biotecnologici nelle piante (AG.P01.006.001) (Modulo)
• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)

Autore CNR

• NUNZIA SCOTTI (Persona)
• MARINA IOVENE (Unità di personale interno)
• TEODORO CARDI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Nuclear and cytoplasmic genome composition of Solanum bulbocastanum (+) S. tuberosum somatic hybrids." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• TEODORO CARDI (Unità di personale interno)
• MARINA IOVENE (Unità di personale interno)
• NUNZIA SCOTTI (Persona)

Prodotto

• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)
• Ottenimento di nuovi prodotti per la salute, l'alimentazione e l'industria mediante interventi genetici e biotecnologici nelle piante (AG.P01.006.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Genome (Rivista)

Insieme di parole chiave di

• Keywords of "Nuclear and cytoplasmic genome composition of Solanum bulbocastanum (+) S. tuberosum somatic hybrids." (Insieme di parole chiave)