ZebraBeat: a flexible platform for the analysis of the cardiac rate in zebrafish embryos (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• ZebraBeat: a flexible platform for the analysis of the cardiac rate in zebrafish embryos (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Alternative label

• Elisa De Luca 1, Gian Maria Zaccaria 2, Marwa Hadhoud 3, Giovanna Rizzo 4, Raffaele Ponzini 5, Umberto Morbiducci 2 & Massimo Mattia Santoro 1,6 (2014)
  ZebraBeat: a flexible platform for the analysis of the cardiac rate in zebrafish embryos
  in Scientific reports (Nature Publishing Group)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Elisa De Luca 1, Gian Maria Zaccaria 2, Marwa Hadhoud 3, Giovanna Rizzo 4, Raffaele Ponzini 5, Umberto Morbiducci 2 & Massimo Mattia Santoro 1,6 (literal)

Pagina inizio

• 4898 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 4 (literal)

Rivista

• Scientific reports (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Department of Molecular Biotechnology and Health Sciences and Molecular BiotechnologyCenter, University of Torino, Turin, Italy, 2. Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin, Italy, 3. Faculty of Engineering, Helwan University, Helwan, Egypt, 4. Institute of Bioimaging and Molecular Physiology, CNR, Milan, Italy, 5. SuperComputing Applications and Innovation Department, CINECA, Milan, Italy, 6Vesalius Research Center, VIB, KU Leuven, Leuven, Belgium. (literal)

Titolo

• ZebraBeat: a flexible platform for the analysis of the cardiac rate in zebrafish embryos (literal)

Abstract

• Heartbeat measurement is important in assesssing cardiac function because variations in heart rhythm can be the cause as well as an effect of hidden pathological heart conditions. Zebrafish (Danio rerio) has emerged as one of the most useful model organisms for cardiac research. Indeed, the zebrafish heart is easily accessible for optical analyses without conducting invasive procedures and shows anatomical similarity to the human heart. In this study, we present a non-invasive, simple, cost-effective process to quantify the heartbeat in embryonic zebrafish. To achieve reproducibility, high throughput and flexibility (i.e., adaptability to any existing confocal microscope system and with a user-friendly interface that can be easily used by researchers), we implemented this method within a software program. We show here that this platform, called ZebraBeat, can successfully detect heart rate variations in embryonic zebrafish at various developmental stages, and it can record cardiac rate fluctuations induced by factors such as temperature and genetic- and chemical-induced alterations. Applications of this methodology may include the screening of chemical libraries affecting heart rhythm and the identification of heart rhythm variations in mutants from large-scale forward genetic screens. (literal)

Prodotto di

• Tecniche di elaborazione dati e immagini (ME.P06.005.002) (Modulo)
• Institute of molecular bioimaging and physiology (IBFM) (Istituto)

Autore CNR

• GIOVANNA RIZZO (Unità di personale interno)

Incoming links:

Autore CNR di

• GIOVANNA RIZZO (Unità di personale interno)

Prodotto

• Institute of molecular bioimaging and physiology (IBFM) (Istituto)
• Tecniche di elaborazione dati e immagini (ME.P06.005.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Scientific reports (Rivista)