Functional screening of plant stress-related cDNAs by random over-expression in Escherichia coli (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Functional screening of plant stress-related cDNAs by random over-expression in Escherichia coli (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.plantsci.2005.12.011 (literal)

Alternative label

• Massarelli I., Cioffi R., Batelli G., De Palma M., Costa A., Grillo S., Leone A. (2006)
  Functional screening of plant stress-related cDNAs by random over-expression in Escherichia coli
  in Plant science (Limerick)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Massarelli I., Cioffi R., Batelli G., De Palma M., Costa A., Grillo S., Leone A. (literal)

Pagina inizio

• 880 (literal)

Pagina fine

• 888 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 170 (literal)

Rivista

• Plant science (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 9 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Institute of Plant Genetics, Research Division of Portici 7 Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Salerno (literal)

Titolo

• Functional screening of plant stress-related cDNAs by random over-expression in Escherichia coli (literal)

Abstract

• A functional screening, based on random over-expression of a plant cDNA library in Escherichia coli, was used to identify plant genes associated to salt tolerance. E. coli cells, transformed with phagemids derived from a cDNA library from Solanum commersonii plantlets, were selected on medium supplemented with 0.8 M NaCl. Sequence analysis of seven cDNAs from salt tolerant bacterial colonies revealed homology either with known plant stress-related genes (dhn2, cpn60b, epoxide hydrolase and others) or with Arabidopsis thaliana putative proteins with unknown functions. Expression of these two novel plant genes was induced by NaCl in S. commersonii and S. tuberosum cells as well as in A. thaliana seedlings, suggesting that the function of their encoded proteins in the cellular response to high salt are conserved across prokaryotes and plant kingdom. N2.1 and N2.2 transcripts were also induced by cold and water stress, but not ABA, revealing that they belong to the ABA- independent stress-responsive gene category. The two unknown cDNAs, as well as dhn2 and cpn60b, were constitutively expressed at higher level in plants of the cold tolerant potato species S. commersonii than in plants of S. tuberosum, the cold susceptible species. These findings support previous evidence that a pre-committed high constitutive transcript level of a set of stress-responsive genes is crucial for plant stress tolerance. (literal)

Prodotto di

• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)
• Genomica e Proteomica per il miglioramento della produttività e della qualità delle piante (AG.P01.007.001) (Modulo)

Autore CNR

• MONICA DE PALMA (Unità di personale interno)
• MARIA STEFANIA GRILLO (Unità di personale interno)
• ANTONELLO COSTA (Persona)

Insieme di parole chiave

• Keywords of "Functional screening of plant stress-related cDNAs by random over-expression in Escherichia coli" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)
• Genomica e Proteomica per il miglioramento della produttività e della qualità delle piante (AG.P01.007.001) (Modulo)

Autore CNR di

• MARIA STEFANIA GRILLO (Unità di personale interno)
• MONICA DE PALMA (Unità di personale interno)
• ANTONELLO COSTA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Plant science (Rivista)

Insieme di parole chiave di

• Keywords of "Functional screening of plant stress-related cDNAs by random over-expression in Escherichia coli" (Insieme di parole chiave)