Hhex and Cer1 mediate the Sox17 pathway for cardiac mesoderm formation in embryonic stem cells. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Hhex and Cer1 mediate the Sox17 pathway for cardiac mesoderm formation in embryonic stem cells. (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/stem.1695. (literal)

Alternative label

• Liu Y, Kaneda R, Leja TW, Subkhankulova T, Tolmachov O, Minchiotti G, Schwartz RJ, Barahona M, Schneider MD. (2014)
  Hhex and Cer1 mediate the Sox17 pathway for cardiac mesoderm formation in embryonic stem cells.
  in Stem cells
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Liu Y, Kaneda R, Leja TW, Subkhankulova T, Tolmachov O, Minchiotti G, Schwartz RJ, Barahona M, Schneider MD. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• Epub ahead of print (literal)

Rivista

• Stem cells (Rivista)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• From the Center for Cardiovascular Development, Baylor College of Medicine, Houston, TX Institute for Biosciences and Technology, Texas A&M University Health Science Center, Houston, TX British Heart Foundation Centre of Research Excellence, Imperial College London, UK National Heart and Lung Institute, Imperial College London, UK Department of Mathematics, Imperial College London, UK Institute of Genetics and Biophysics \"Adriano Buzzati Traverso\", CNR, Naples, Italy Department of Regenerative Medicine and Advanced Cardiac Therapeutics, Keio University School of Medicine, Tokyo, Japan Department of Biology & Biochemistry, University of Houston, Houston, TX (literal)

Titolo

• Hhex and Cer1 mediate the Sox17 pathway for cardiac mesoderm formation in embryonic stem cells. (literal)

Abstract

• Cardiac muscle differentation in vivo is guided by sequential growth factor signals, including endoderm-derived diffusible factors, impinging on cardiogenic genes in the developing mesoderm. Previously, by RNA interference in AB2.2 mouse embryonic stem cells (ESCs), we identified the endodermal transcription factor Sox17 as essential for Mesp1 induction in primitive mesoderm and subsequent cardiac muscle differentiation. However, downstream effectors of Sox17 remained to be proven functionally. In the present study, we used genome-wide profiling of Sox17-dependent genes in AB2.2 cells, RNA interference, chromatin immunoprecipitation, and luciferase reporter genes to dissect this pathway. Sox17 was required not only for Hhex (a second endodermal transcription factor) but also for Cer1, a growth factor inhibitor from endoderm that, like Hhex, controls mesoderm patterning in Xenopus toward a cardiac fate. Suppressing Hhex or Cer1 blocked cardiac myogenesis, though at a later stage than induction of Mesp1/2. Hhex was required but not sufficient for Cer1 expression. Over-expression of Sox17 induced endogenous Cer1 and sequence-specific transcription of a Cer1 reporter gene. Forced expression of Cer1 was sufficient to rescue cardiac differentiation in Hhex-deficient cells. Thus, Hhex and Cer1 are indispensable components of the Sox17 pathway for cardiopoiesis in mESCs, acting at a stage downstream from Mesp1/2 (literal)

Prodotto di

• Institute of genetics and biophysics \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Identificazione di regolatori del differenziamento, della motilità e dell'apoptosi delle cellule staminali (SV.P15.008.001) (Modulo)

Autore CNR

• GABRIELLA MINCHIOTTI (Unità di personale interno)

Incoming links:

Prodotto

• Institute of genetics and biophysics \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Identificazione di regolatori del differenziamento, della motilità e dell'apoptosi delle cellule staminali (SV.P15.008.001) (Modulo)

Autore CNR di

• GABRIELLA MINCHIOTTI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Stem cells (Rivista)