Variety identification in maize lines via capillary electrophoresis of zeins in isoelectric acidic buffers (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Variety identification in maize lines via capillary electrophoresis of zeins in isoelectric acidic buffers (Articolo in rivista) (literal)

Anno

• 1999-01-01T00:00:00+01:00 (literal)

Alternative label

• Erna Olivieri1, Angelo Viotti2, Massimiliano Lauria2, Ernesto Simò-Alfonso3, Pier Giorgio Righetti1 (1999)
  Variety identification in maize lines via capillary electrophoresis of zeins in isoelectric acidic buffers
  in Electrophoresis (Weinh., Internet)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Erna Olivieri1, Angelo Viotti2, Massimiliano Lauria2, Ernesto Simò-Alfonso3, Pier Giorgio Righetti1 (literal)

Pagina inizio

• 1595 (literal)

Pagina fine

• 1604 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://onlinelibrary.wiley.com/journal/10.1002/%28ISSN%291522-2683 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 20 (literal)

Rivista

• Electrophoresis (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1 Department of Agricultural and Industrial Biotechnologies, University of Verona, Verona, Italy 2 Istituto Biosintesi Vegetali CNR, Milano, Italy 3 Universitat de Valencia, Departamento de Quimica Analitica, Burjassot (Valencia), Spain (literal)

Titolo

• Variety identification in maize lines via capillary electrophoresis of zeins in isoelectric acidic buffers (literal)

Abstract

• Zeins (the prolamins or seed storage proteins in maize) have been used to characterize and identify different genotypes. Zeins were fractionated by capillary zone electrophoresis in acidic, amphoteric buffers, which represent a medium of moderate conductivity and are thus compatible with higher voltage gradients. The running buffer consisted of 40 mM isoelectric aspartic acid, in presence of 6 M urea and 0.5% hydroxyethyl cellulose (apparent pH: 3.8; pI in the absence of urea: 2.77). Thirty-one different zein peaks were mapped out of a total of 21 different maize genotypes. Each of them typically exhibited seven to twelve peaks, with some genotypes showing up to 20 zein bands. Due to slightly changing elution times, caused by a lack of reproducibility of the electroendoosmotic flow in uncoated silica surfaces, correct peak assignment and alignment among different runs was obtained by multivariate statistical analysis. The present method compares well, both in resolution and total number of peaks, with current protocols adopted for screening of maize inbreds, which consist of isoelectric focusing in agarose gels (literal)

Prodotto di

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)

Autore CNR

• MASSIMILIANO LAURIA (Unità di personale interno)
• ANGELO VIOTTI (Unità di personale esterno)

Insieme di parole chiave

• Parole chiave di "Variety identification in maize lines via capillary electrophoresis of zeins in isoelectric acidic buffers" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)

Autore CNR di

• MASSIMILIANO LAURIA (Unità di personale interno)
• ANGELO VIOTTI (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Electrophoresis (Rivista)

Insieme di parole chiave di

• Parole chiave di "Variety identification in maize lines via capillary electrophoresis of zeins in isoelectric acidic buffers" (Insieme di parole chiave)