Age- and calorie restriction-related changes in rat brain mitochondrial DNA and TFAM binding (Articolo in rivista)

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Label
  • Age- and calorie restriction-related changes in rat brain mitochondrial DNA and TFAM binding (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/s11357-012-9465-z (literal)
Alternative label
  • Anna Picca, Flavio Fracasso, Vito Pesce, Palmiro Cantatore, Anna-Maria Joseph, Christiaan Leeuwenburgh, Maria Nicola Gadaleta, Angela Maria Serena Lezza (2013)
    Age- and calorie restriction-related changes in rat brain mitochondrial DNA and TFAM binding
    in Age (Omaha); Springer, Dordrecht (Paesi Bassi)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Anna Picca, Flavio Fracasso, Vito Pesce, Palmiro Cantatore, Anna-Maria Joseph, Christiaan Leeuwenburgh, Maria Nicola Gadaleta, Angela Maria Serena Lezza (literal)
Pagina inizio
  • 1607 (literal)
Pagina fine
  • 1620 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 35 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 5 (literal)
Note
  • Scopus (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • -Department of Biosciences, Biotechnologies and Pharmacological Sciences, University of Bari, Via Orabona, 4, 70125 Bari, Italy -Institute of Biomembranes and Bioenergetics, CNR-National Research Council of Italy, Via Amendola, 165/A, 70126 Bari, Italy -Division of Biology of Aging, Department of Aging and Geriatric Research, Institute on Aging, University of Florida, Gainesville, FL, USA (literal)
Titolo
  • Age- and calorie restriction-related changes in rat brain mitochondrial DNA and TFAM binding (literal)
Abstract
  • Aging markedly affects mitochondrial biogenesis and functions particularly in tissues highly dependent on the organelle's bioenergetics capability such as the brain's frontal cortex. Calorie restriction (CR) diet is, so far, the only intervention able to delay or prevent the onset of several age-related alterations in different organisms. We determined the contents of mitochondrial transcription factor A (TFAM), mitochondrial DNA (mtDNA), and the 4.8-kb mtDNA deletion in the frontal cortex from young (6-monthold) and aged (26-month-old), ad libitum-fed (AL) and calorie-restricted (CR), rats. We found a 70 % increase in TFAM amount, a 25 % loss in mtDNA content, and a 35 % increase in the 4.8-kb deletion content in the aged AL animals with respect to the young rats. TFAM-specific binding to six mtDNA regions was analyzed by mtDNA immunoprecipitation and semiquantitative polymerase chain reaction (PCR), showing a marked age-related decrease. Quantitative realtime PCR at two subregions involved in mtDNA replication demonstrated, in aged AL rats, a remarkable decrease (60-70 %) of TFAM-bound mtDNA. The decreased TFAM binding is a novel finding that may explain the mtDNA loss in spite of the compensatory TFAM increased amount. In aged CR rats, TFAM amount increased and mtDNA content decreased with respect to young rats' values, but the extent of the changes was smaller than in aged AL rats. Attenuation of the age-related effects due to the diet in the CR animals was further evidenced by the unchanged content of the 4.8-kb deletion with respect to that of young animals and by the partial prevention of the age-related decrease in TFAM binding to mtDNA. (literal)
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