http://www.cnr.it/ontology/cnr/individuo/prodotto/ID276406
Age- and calorie restriction-related changes in rat brain mitochondrial DNA and TFAM binding (Articolo in rivista)
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- Label
- Age- and calorie restriction-related changes in rat brain mitochondrial DNA and TFAM binding (Articolo in rivista) (literal)
- Anno
- 2013-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1007/s11357-012-9465-z (literal)
- Alternative label
Anna Picca, Flavio Fracasso, Vito Pesce,
Palmiro Cantatore, Anna-Maria Joseph,
Christiaan Leeuwenburgh, Maria Nicola Gadaleta,
Angela Maria Serena Lezza (2013)
Age- and calorie restriction-related changes in rat brain mitochondrial DNA and TFAM binding
in Age (Omaha); Springer, Dordrecht (Paesi Bassi)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Anna Picca, Flavio Fracasso, Vito Pesce,
Palmiro Cantatore, Anna-Maria Joseph,
Christiaan Leeuwenburgh, Maria Nicola Gadaleta,
Angela Maria Serena Lezza (literal)
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- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
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- -Department of Biosciences, Biotechnologies and Pharmacological Sciences, University of Bari, Via Orabona, 4, 70125 Bari, Italy
-Institute of Biomembranes and Bioenergetics, CNR-National Research Council of Italy, Via Amendola, 165/A, 70126 Bari, Italy
-Division of Biology of Aging, Department of Aging and Geriatric Research, Institute on Aging, University of Florida, Gainesville, FL, USA (literal)
- Titolo
- Age- and calorie restriction-related changes in rat brain mitochondrial DNA and TFAM binding (literal)
- Abstract
- Aging markedly affects mitochondrial biogenesis
and functions particularly in tissues highly
dependent on the organelle's bioenergetics capability
such as the brain's frontal cortex. Calorie restriction
(CR) diet is, so far, the only intervention able to delay
or prevent the onset of several age-related alterations
in different organisms. We determined the contents of
mitochondrial transcription factor A (TFAM), mitochondrial
DNA (mtDNA), and the 4.8-kb mtDNA
deletion in the frontal cortex from young (6-monthold)
and aged (26-month-old), ad libitum-fed (AL) and
calorie-restricted (CR), rats. We found a 70 % increase
in TFAM amount, a 25 % loss in mtDNA content, and
a 35 % increase in the 4.8-kb deletion content in the
aged AL animals with respect to the young rats.
TFAM-specific binding to six mtDNA regions was
analyzed by mtDNA immunoprecipitation and semiquantitative
polymerase chain reaction (PCR), showing
a marked age-related decrease. Quantitative realtime
PCR at two subregions involved in mtDNA replication
demonstrated, in aged AL rats, a remarkable
decrease (60-70 %) of TFAM-bound mtDNA. The
decreased TFAM binding is a novel finding that may
explain the mtDNA loss in spite of the compensatory
TFAM increased amount. In aged CR rats, TFAM
amount increased and mtDNA content decreased with
respect to young rats' values, but the extent of the
changes was smaller than in aged AL rats. Attenuation
of the age-related effects due to the diet in the CR
animals was further evidenced by the unchanged content
of the 4.8-kb deletion with respect to that of young
animals and by the partial prevention of the age-related
decrease in TFAM binding to mtDNA. (literal)
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