Application of direct HPTLC-MALDI for the qualitative and quantitative profiling of neutral and acidic glycosphingolipids: the case of NEU3 overexpressing C2C12 murine myoblasts (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Application of direct HPTLC-MALDI for the qualitative and quantitative profiling of neutral and acidic glycosphingolipids: the case of NEU3 overexpressing C2C12 murine myoblasts (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/elps.201300474 (literal)

Alternative label

• Enrica Torretta 1; Michele Vasso 2; Chiara Fania 1; Daniele Capitanio 1; Sonia Bergante 1,3; Marco Piccoli 1,3; Guido Tettamanti 3; Luigi Anastasia 1,3; Cecilia Gelfi *1,2 (2014)
  Application of direct HPTLC-MALDI for the qualitative and quantitative profiling of neutral and acidic glycosphingolipids: the case of NEU3 overexpressing C2C12 murine myoblasts
  in Electrophoresis (Weinh., Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Enrica Torretta 1; Michele Vasso 2; Chiara Fania 1; Daniele Capitanio 1; Sonia Bergante 1,3; Marco Piccoli 1,3; Guido Tettamanti 3; Luigi Anastasia 1,3; Cecilia Gelfi *1,2 (literal)

Pagina inizio

• 1319 (literal)

Pagina fine

• 1328 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• Pubblicato on-line: 23 Dicembre 2013. IF 2012: 3.261 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 35 (literal)

Rivista

• Electrophoresis (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 9 (literal)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1 Department of Biomedical Sciences for Health, University of Milan, Segrate (Milan), Italy 2 Institute of Molecular Bioimaging and Physiology (IBFM), CNR, Cefalù (Palermo) Segrate (Milan), Italy. 3 Laboratory of Stem Cells for Tissue Engineering, IRCCS Policlinico San Donato (Milan), Italy (literal)

Titolo

• Application of direct HPTLC-MALDI for the qualitative and quantitative profiling of neutral and acidic glycosphingolipids: the case of NEU3 overexpressing C2C12 murine myoblasts (literal)

Abstract

• Glycosphingolipids (GSLs) are a class of ubiquitous lipids characterized by a wide structural repertoire and a variety of functional implications. Importantly, altered levels have been correlated with different diseases, suggesting their crucial role in health. Conventional methods for the characterization and quantification are based on HPTLC separation and comparison with the migration distance of standard samples or on mass spectrometry. We set up and herein report the application of an ImagePrep method for glycosphingolipids qualitative and quantitative profiling through direct HPTLC-MALDI with particular application to wild-type and NEU3- overexpressing C2C12 myoblasts. Lipids were analysed by HPTLC, coupled with MALDI-TOF and the resulting GSLs profiles were compared to the [3 H]sphingolipids HPTLC pattern obtained after metabolic radiolabelling. GSLs detection by HPTLC-MALDI was optimized by testing different methods for matrix delivery and by performing quantitative analyses using serial dilutions of GSLs standards. Through this approach an accurate analysis of each variant of neutral and acidic GSLs, including the detection of different fatty-acid chain variants for each GSL, was provided and these results demonstrate that HPTLC-MALDI is an easy and high-throughput analytical method for GSLs profiling suggesting its use for an early detection of markers in different diseases, including cancer and heart ischemia (literal)

Prodotto di

• Institute of molecular bioimaging and physiology (IBFM) (Istituto)
• Proteomica differenziale di tessuti patologici e fluidi biologici per la comprensione della biologia dei sistemi (ME.P06.026.002) (Modulo)

Autore CNR

• MICHELE VASSO (Unità di personale interno)
• CECILIA GELFI (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "Application of direct HPTLC-MALDI for the qualitative and quantitative profiling of neutral and acidic glycosphingolipids: the case of NEU3 overexpressing C2C12 murine myoblasts" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular bioimaging and physiology (IBFM) (Istituto)
• Proteomica differenziale di tessuti patologici e fluidi biologici per la comprensione della biologia dei sistemi (ME.P06.026.002) (Modulo)

Autore CNR di

• MICHELE VASSO (Unità di personale interno)
• CECILIA GELFI (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Electrophoresis (Rivista)

Insieme di parole chiave di

• Keywords of "Application of direct HPTLC-MALDI for the qualitative and quantitative profiling of neutral and acidic glycosphingolipids: the case of NEU3 overexpressing C2C12 murine myoblasts" (Insieme di parole chiave)