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Upgraded diagnostic value of Gen-Probe PACE 2 assay for detection of Chlamydia trachomatis infection. (Articolo in rivista)

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Upgraded diagnostic value of Gen-Probe PACE 2 assay for detection of Chlamydia trachomatis infection. (Articolo in rivista) (literal)

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Robuffo I; Fazii P; Rulli A; Di Nicola M; Toniato E; Di Rienzo M; Cosentino L; Gambi A; Castellani ML; Martinotti S. (2008)
Upgraded diagnostic value of Gen-Probe PACE 2 assay for detection of Chlamydia trachomatis infection.
in Journal of Biological Regulators & Homeostatic Agents (Testo stamp.); Biolife SAS, Silva Marina (TE) (Italia)
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Robuffo I; Fazii P; Rulli A; Di Nicola M; Toniato E; Di Rienzo M; Cosentino L; Gambi A; Castellani ML; Martinotti S. (literal)

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Istituto di Genetica Molecolare, CNR, Sezione di Chieti, Chieti; Laboratorio Analisi Chimico_Cliniche e Microbiologia, P.O. \"Spirito Santo\", Pescara; Laboratorio Analisi Chimico-Cliniche e Microbiologia, Ospedale Clinicizzato \" SS Annunziata\", Chieti; Dipartimento di Scienze Biomediche, Laboratorio di Biostatistica, Università \"G. d'Annunzio\", Chieti; Dipartimento di Oncologia e Neuroscienze, Patologia Generale, Università \"G. d'Annunzio\", Chieti; Department of Medicine and Science of Ageing, University of Chieti, Chieti, Center of Excellenge on Aging, CRC, G. d'Annunzio Foundation, University of Chieti, Chieti, Italy. (literal)

Titolo

Upgraded diagnostic value of Gen-Probe PACE 2 assay for detection of Chlamydia trachomatis infection. (literal)

Abstract

In this study, we evaluate the performance of a nucleic acid amplification assay, COBAS AMPLICOR (Roche Molecular systems) (PCR), compared to non-amplified DNA probe assay PACE2 (Gen-Probe Inc.) for the detection of C. trachomatis in a total of 2,916 samples (2,114 females and 802 males) consecutively collected in two different clinical pathology laboratories, over a period of three years. In the females, the endocervical swabs showed a similar range of detection when using the two different methods: out of 1,581 females processed with PACE 2, 1.4% (2005), 0.9% (2006), 0.5% (2007), resulted positive for C. trachomatis; out of 533 females processed with PCR, 1.3% (2005), 1.5% (2006) and 1.2% (2007), resulted positive. However, in the male subjects we found an increased positivity of Chlamydia detection on urethral swabs by using PACE 2: 4.8% (2005), 1.9% (2006) and 2.9% (2007), compared to urine specimen processed by PCR: 1% (2005), 1.4% (2006) and 0% (2007). Even if PCR should be considered a most promising tool for routine diagnosis of Chlamydia infection, Gen Probe allowed us to better identify Chlamydia trachomatis (in 4.8% of urethral swabs compared to urine) leading to a hypothesis that extracellular EB forms of Chlamydia could be absent in urine in persistent infectious. (literal)

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