http://www.cnr.it/ontology/cnr/individuo/prodotto/ID274138

Differential patterns of Notch1-4 receptor expression are markers of glioma cell differentiation. (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Differential patterns of Notch1-4 receptor expression are markers of glioma cell differentiation. (Articolo in rivista) (literal)

Anno

2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1093/neuonc/not168 (literal)

Alternative label

Dell'Albani P., Rodolico M, Pellitteri R., Tricarichi E., Torrisi S.A., D'Antoni S., Zappia M., Albanese V., Caltabiano R., Platania N., Aronica E.,and Catania M.V. (2014)
Differential patterns of Notch1-4 receptor expression are markers of glioma cell differentiation.
in Neuro-oncology (Online); Oxford University Press Inc., Cary (Stati Uniti d'America)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Dell'Albani P., Rodolico M, Pellitteri R., Tricarichi E., Torrisi S.A., D'Antoni S., Zappia M., Albanese V., Caltabiano R., Platania N., Aronica E.,and Catania M.V. (literal)

Pagina inizio

204 (literal)

Pagina fine

216 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

16 (literal)

Rivista

Neuro-oncology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

13 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

2 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Institute of Neurological Sciences, CNR, Via P.Gaifami, 18 - 95126 Catania, Italy (P.D, M.R, R.P., E.T., S.A.T, S.D., V.A., M.V.C.) Dept. Chemical Sciences, Section of Biochemistry and Molecular Biology, University of Catania, Viale A. Doria, 6 - 95125, Catania, Italy (E.T.) Dept. of Clinical and Molecular Biomedicine, Section of Pharmacology and Biochemistry, University of Catania, Viale A. Doria, 6 - 95125 Catania, Italy (S.A.T) Dept. of Neurosciences, University of Catania, Via Santa Sofia, 86 - 95125, Catania, Italy. (M.Z., V.A., N.P.) Dept. G.F. Ingrassia, Section of Anatomic Pathology, University of Catania, 87 Santa Sofia Street, 95123 Catania, Italy (R.C) Dept. of (Neuro)Pathology, H0-122, Academisch Medisch Centrum, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands (EA) Epilepsy Institute of Netherlands Foundation (Stichting Epilepsie Instelligen Nederland, SEIN), Heemstede, The Netherlands. (EA) IRCCS Oasi Maria SS, Via Conte Ruggero, 73 - 94018 Troina (EN), Italy (M.V.C.) (literal)

Titolo

Differential patterns of Notch1-4 receptor expression are markers of glioma cell differentiation. (literal)

Abstract

Background. Notch signaling is deregulated in human gliomas and may play a role in their malignancy. However, the role of each Notch receptor in glioma cell differentiation and progression is not clear. We examined the expression pattern of Notch receptors and compared it with differentiation markers in gliomas' i) cell lines, ii) primary human cultures, and iii) biopsies of different grade. Furthermore, the effects of a ?-secretase-inhibitor (GSI) on cell survival were assessed. Methods. Notch receptors and markers of cellular differentiation were analyzed by Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) , Western blotting, immunohistochemistry and immunocytochemistry. GSI sensitivity was assessed in both cell lines and primary cultures grown as monolayer or tumorspheres, by MTT assay. Results. In cell lines Notch1 and Notch2/4 levels paralleled those of GFAP and vimentin, respectively. In human gliomas and in primary cultures Notch1 was moderate/strong in low grade, but weak in GBMs. Conversely, Notch4 increased from AstrII to GBMs. Primary GBM cultures grown in serum (monolayer) showed moderate/high levels of CD133, nestin, vimentin and Notch4 and very low levels of GFAP and Notch1, which were reduced in tumorspheres. This effect was drastic for Notch4. GSI reduced cell survival with stronger effect in serum, whilst human primary cultures showed different sensitivity. Conclusion. Data from cell lines and human gliomas suggest a correlation between expression of Notch receptors and cell differentiation. Namely, Notch1 and Notch4 are markers of differentiated and less differentiated glioma cells, respectively. We propose Notch receptors as markers of glioma grading and possible prognostic factors. (literal)

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