Ligand Binding Promiscuity of Human Liver Fatty Acid Binding Protein: Structural and Dynamic Insights from an Interaction Study with Glycocholate and Oleate (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Ligand Binding Promiscuity of Human Liver Fatty Acid Binding Protein: Structural and Dynamic Insights from an Interaction Study with Glycocholate and Oleate (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/cbic.201300156 (literal)

Alternative label

• Favretto F.; Assfalg M.; Gallo M.; Cicero D.O.; D'Onofrio M.;Molinari H. (2013)
  Ligand Binding Promiscuity of Human Liver Fatty Acid Binding Protein: Structural and Dynamic Insights from an Interaction Study with Glycocholate and Oleate
  in ChemBioChem (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Favretto F.; Assfalg M.; Gallo M.; Cicero D.O.; D'Onofrio M.;Molinari H. (literal)

Pagina inizio

• 1807 (literal)

Pagina fine

• 1819 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 14 (literal)

Rivista

• ChemBioChem (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 14 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• D'Onofrio, M (Reprint Author), Univ Verona, NMR Lab, Dept Biotechnol, Str Grazie 15, I-37134 Verona, Italy. Favretto, Filippo; Assfalg, Michael; D'Onofrio, Mariapina, Univ Verona, NMR Lab, Dept Biotechnol, I-37134 Verona, Italy. Gallo, Mariana, Consejo Nacl Invest Cient & Tecn, Fdn Inst Leloir, IIBBA, RA-1033 Buenos Aires, DF, Argentina. Cicero, Daniel Oscar, Univ Roma Tor Vergata, Dept Chem Sci & Technol, I-00133 Rome, Italy. Molinari, Henriette, ISMAC CNR, Lab NMR, I-20133 Milan, Italy. (literal)

Titolo

• Ligand Binding Promiscuity of Human Liver Fatty Acid Binding Protein: Structural and Dynamic Insights from an Interaction Study with Glycocholate and Oleate (literal)

Abstract

• Human liver fatty acid binding protein (hL-FABP) has been reported to act as an intracellular shuttle of lipid molecules, thus playing a central role in systemic metabolic homeostasis. The involvement of hL-FABP in the transport of bile salts has been postulated but scarcely investigated. Here we describe a thorough NMR investigation of glycocholate (GCA) binding to hL-FABP. The protein molecule bound a single molecule of GCA, in contrast to the 1: 2 stoichiometry observed with fatty acids. GCA was found to occupy the large internal cavity of hL-FABP, without requiring major conformational rearrangement of the protein backbone; rather, this led to increased stability, similar to that estimated for the hL-FABP: oleate complex. Fast-timescale dynamics appeared not to be significantly perturbed in the presence of ligands. Slow motions (unlike for other proteins of the family) were retained or enhanced upon binding, consistent with a requirement for structural plasticity for promiscuous recognition. (literal)

Prodotto di

• Istituto per lo studio delle macromolecole (ISMAC) (Istituto)
• Strutturistica NMR di proteine legate a processi patologici (PM.P01.026.003) (Modulo)

Autore CNR

• LAURA GIUDITTA RAGONA (Unità di personale interno)
• HENRIETTE MOLINARI (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "Ligand Binding Promiscuity of Human Liver Fatty Acid Binding Protein: Structural and Dynamic Insights from an Interaction Study with Glycocholate and Oleate" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto per lo studio delle macromolecole (ISMAC) (Istituto)
• Strutturistica NMR di proteine legate a processi patologici (PM.P01.026.003) (Modulo)

Autore CNR di

• LAURA GIUDITTA RAGONA (Unità di personale interno)
• HENRIETTE MOLINARI (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• ChemBioChem (Rivista)

Insieme di parole chiave di

• Keywords of "Ligand Binding Promiscuity of Human Liver Fatty Acid Binding Protein: Structural and Dynamic Insights from an Interaction Study with Glycocholate and Oleate" (Insieme di parole chiave)