Protection of Cells against Oxidative Stress by Nanomolar Levels of Hydroxyflavones Indicates a New Type of Intracellular Antioxidant Mechanism (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Protection of Cells against Oxidative Stress by Nanomolar Levels of Hydroxyflavones Indicates a New Type of Intracellular Antioxidant Mechanism (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1371/journal.pone.0060796 (literal)

Alternative label

• Lombardo, Emanuele; Sabellico, Cristian; Hajek, Jan; Stankova, Veronika; Filipsky, Tomas; Balducci, Valentina; De Vito, Paolo; Leone, Stefano; Bavavea, Eugenia I.; Silvestri, Ilaria Proietti; Righi, Giuliana; Luly, Paolo; Saso, Luciano; Bovicelli, Paolo; Pedersen, Jens Z.; Incerpi, Sandra (2013)
  Protection of Cells against Oxidative Stress by Nanomolar Levels of Hydroxyflavones Indicates a New Type of Intracellular Antioxidant Mechanism
  in PloS one
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Lombardo, Emanuele; Sabellico, Cristian; Hajek, Jan; Stankova, Veronika; Filipsky, Tomas; Balducci, Valentina; De Vito, Paolo; Leone, Stefano; Bavavea, Eugenia I.; Silvestri, Ilaria Proietti; Righi, Giuliana; Luly, Paolo; Saso, Luciano; Bovicelli, Paolo; Pedersen, Jens Z.; Incerpi, Sandra (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0060796 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 8 (literal)

Rivista

• PloS one (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• e60796 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 4 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Univ Roma Tre, Dept Sci, Rome, Italy Charles Univ Prague, Fac Pharm Hradec Kralove, Dept Biochem Sci, Prague, Czech Republic Charles Univ Prague, Fac Pharm Hradec Kralove, Dept Pharmacol & Toxicol, Prague, Czech Republic Univ Roma Tor Vergata, Dept Biol, I-00173 Rome, Italy Univ Athens, Fac Pharm, Dept Med Chem, GR-10679 Athens, Greece Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy CNR, Inst Biomol Chem, Rome, Italy Univ Roma La Sapienza, Dept Physiol & Pharmacol Vittorio Erspamer, Rome, Italy (literal)

Titolo

• Protection of Cells against Oxidative Stress by Nanomolar Levels of Hydroxyflavones Indicates a New Type of Intracellular Antioxidant Mechanism (literal)

Abstract

• Natural polyphenol compounds are often good antioxidants, but they also cause damage to cells through more or less specific interactions with proteins. To distinguish antioxidant activity from cytotoxic effects we have tested four structurally related hydroxyflavones (baicalein, mosloflavone, negletein, and 5,6-dihydroxyflavone) at very low and physiologically relevant levels, using two different cell lines, L-6 myoblasts and THP-1 monocytes. Measurements using intracellular fluorescent probes and electron paramagnetic resonance spectroscopy in combination with cytotoxicity assays showed strong antioxidant activities for baicalein and 5,6-dihydroxyflavone at picomolar concentrations, while 10 nM partially protected monocytes against the strong oxidative stress induced by 200 mu M cumene hydroperoxide. Wide range dose-dependence curves were introduced to characterize and distinguish the mechanism and targets of different flavone antioxidants, and identify cytotoxic effects which only became detectable at micromolar concentrations. Analysis of these dose-dependence curves made it possible to exclude a protein-mediated antioxidant response, as well as a mechanism based on the simple stoichiometric scavenging of radicals. The results demonstrate that these flavones do not act on the same radicals as the flavonol quercetin. Considering the normal concentrations of all the endogenous antioxidants in cells, the addition of picomolar or nanomolar levels of these flavones should not be expected to produce any detectable increase in the total cellular antioxidant capacity. The significant intracellular antioxidant activity observed with 1 pM baicalein means that it must be scavenging radicals that for some reason are not eliminated by the endogenous antioxidants. The strong antioxidant effects found suggest these flavones, as well as quercetin and similar polyphenolic antioxidants, at physiologically relevant concentrations act as redox mediators to enable endogenous antioxidants to reach and scavenge different pools of otherwise inaccessible radicals. (literal)

Prodotto di

• Design e sintesi di radical scavengers aventi caratteristiche autoassemblanti. (PM.P01.025.003) (Modulo)
• Antiossidanti per uso farmaceutico, cosmetico e alimentare (PM.P01.011.006) (Modulo)
• Istituto di chimica biomolecolare (ICB) (Istituto)

Autore CNR

• PAOLO BOVICELLI (Unità di personale interno)
• GIULIANA RIGHI (Persona)

Insieme di parole chiave

• Keywords of "Protection of Cells against Oxidative Stress by Nanomolar Levels of Hydroxyflavones Indicates a New Type of Intracellular Antioxidant Mechanism" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• GIULIANA RIGHI (Persona)
• PAOLO BOVICELLI (Unità di personale interno)

Prodotto

• Istituto di chimica biomolecolare (ICB) (Istituto)
• Design e sintesi di radical scavengers aventi caratteristiche autoassemblanti. (PM.P01.025.003) (Modulo)
• Antiossidanti per uso farmaceutico, cosmetico e alimentare (PM.P01.011.006) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• PloS one (Rivista)

Insieme di parole chiave di

• Keywords of "Protection of Cells against Oxidative Stress by Nanomolar Levels of Hydroxyflavones Indicates a New Type of Intracellular Antioxidant Mechanism" (Insieme di parole chiave)