http://www.cnr.it/ontology/cnr/individuo/prodotto/ID273192

Tailoring odorant-binding protein coatings characteristics for surface acoustic wave biosensor development (Articolo in rivista)

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Tailoring odorant-binding protein coatings characteristics for surface acoustic wave biosensor development (Articolo in rivista) (literal)

Anno

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Alternative label

DI PIETROANTONIO F., BENETTI M., DINCA V., CANNATA' D., VERONA E., D'AURIA S., DINESCU M. (2014)
Tailoring odorant-binding protein coatings characteristics for surface acoustic wave biosensor development
in Applied surface science; ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, AMSTERDAM (Paesi Bassi)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

DI PIETROANTONIO F., BENETTI M., DINCA V., CANNATA' D., VERONA E., D'AURIA S., DINESCU M. (literal)

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a Institute of Acoustics and Sensors \"O. M. Corbino\", National Research Council of Italy, Via del Fosso del Cavaliere 100, 00133 Rome, Italy b National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, PO Box MG-16, 077125 Magurele, Romania c Institute for Photonics and Nanotechnologies, National Research Council of Italy, Via del Cineto Romano 42, 00156 Rome, Italy d Institute of Protein Biochemistry, National Research Council of Italy, Via Pietro Castellino 111, 80131 Naples, Italy (literal)

Titolo

Tailoring odorant-binding protein coatings characteristics for surface acoustic wave biosensor development (literal)

Abstract

In this study, wild type bovine odorant-binding proteins (wtbOBPs) were deposited by matrix-assisted pulsed laser evaporation (MAPLE) and utilized as active material on surface acoustic wave (SAW) biosensors. Fourier transform infrared spectroscopy (FTIR), and atomic force microscopy (AFM) were used to determine the chemical, morphological characteristics of the protein thin films. The FTIR data demonstrates that the functional groups of wtbOBPs do not suffer significant changes in the MAPLE-deposited films when compared to the reference one. The topographical studies show that the homogeneity, density and the roughness of the coatings are related mainly to the laser parameters (fluence and number of pulses). SAW biosensor responses to different concentrations of R-(-)-1-octen-3-ol (octenol) and R-(-)-carvone (carvone) were evaluated. The obtained sensitivities, achieved through the optimization of deposition parameters, demonstrated that MAPLE is a promising deposition technique for SAW biosensor implementation. (literal)

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