Autofluorescence spectroscopy of rat liver during experimental transplantation procedure. An approach for hepatic metabolism assessment. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Autofluorescence spectroscopy of rat liver during experimental transplantation procedure. An approach for hepatic metabolism assessment. (Articolo in rivista) (literal)

Anno

• 2005-01-01T00:00:00+01:00 (literal)

Alternative label

• Croce A.C., Ferrigno A., Vairetti M., Bertone R., Freitas I., Bottiroli G. (2005)
  Autofluorescence spectroscopy of rat liver during experimental transplantation procedure. An approach for hepatic metabolism assessment.
  in Photochemical & photobiological sciences (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Croce A.C., Ferrigno A., Vairetti M., Bertone R., Freitas I., Bottiroli G. (literal)

Pagina inizio

• 583 (literal)

Pagina fine

• 590 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• Articolo ripreso e pubblicizzato dalla rivista internazionale di divulgazione e pubblicizzazione delle novità nel campo della biofotonica e e sue applicazioni in medicina (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 4 (literal)

Rivista

• Photochemical & photobiological sciences (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• Impact Factor = 1.798 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Metodica per valutazione dello stato funzionale del fegato durante le fasi di trapianto; Metodica per valutazione dell’efficacia di procedure di preservazione del fegato durante le fasi di trapianto (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Bertone: Lab. Anatomia Comparata e Citologia, Dip. Biologia Animale, Uni PV; Vairetti, Ferrigno: Dip. Medicina Interna e Terapia, Uni PV. Croce, Bottiroli: IGM-CNR (literal)

Titolo

• Autofluorescence spectroscopy of rat liver during experimental transplantation procedure. An approach for hepatic metabolism assessment. (literal)

Abstract

• Ischemia-reperfusion injury, a major cause of organ metabolic alterations and consequent dysfunction in liver transplantation, could be overcome by optimizing organ preservation procedures. The potential of autofluorescence analysis was investigated with the aim to define parameters suitable for in vivo monitoring tissue functionality. Spectrofluorometric analysis was performed on explanted rat livers during cold storage, under standard (4 degrees C University of Wisconsin medium for 20 h) and purposely damaging (4 degrees C Eurocollins medium for 20, 43 and 72 h) preservation conditions, and reperfusion (rewarming-reoxygenation). For both preservation conditions, cold hypoxia caused a signal amplitude increase, mainly attributable to NAD(P)H, and a spectral shape modification, ascribable to changes in the relative contributions of NAD(P)H and flavins, as a result of the tissue reduced state enhancement. Upon rewarming-reoxygenation the autofluorescence signal decreased with a rate depending on the preservation conditions. The time constant changed according to the extent of the liver functionality impairment, as assessed by conventional biochemical and histochemical analyses, thus providing a parameter exploitable for an in situ, real time monitoring of the efficacy of experimental preservation procedures. (literal)

Prodotto di

• Sviluppi metodologici in citometria e applicazioni alla sperimentazione biomedica; in vitro imaging (ME.P06.010.001) (Modulo)
• Institute of molecular genetics (IGM) (Istituto)

Autore CNR

• ANNA CLETA CROCE (Persona)
• Isabel Freitas (Persona)
• GIOVANNI BOTTIROLI (Persona)

Insieme di parole chiave

• Keywords of "Autofluorescence spectroscopy of rat liver during experimental transplantation procedure. An approach for hepatic metabolism assessment." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• Sviluppi metodologici in citometria e applicazioni alla sperimentazione biomedica; in vitro imaging (ME.P06.010.001) (Modulo)

Autore CNR di

• GIOVANNI BOTTIROLI (Persona)
• ANNA CLETA CROCE (Persona)
• Isabel Freitas (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Photochemical & photobiological sciences (Rivista)

Insieme di parole chiave di

• Keywords of "Autofluorescence spectroscopy of rat liver during experimental transplantation procedure. An approach for hepatic metabolism assessment." (Insieme di parole chiave)