A new monoclonal antibody against DNA ligase I is a suitable marker of cell proliferation in cultured cell and tissue section samples. (Articolo in rivista)

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  • A new monoclonal antibody against DNA ligase I is a suitable marker of cell proliferation in cultured cell and tissue section samples. (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Alternative label
  • Vitolo B., Lidonnici M.R., Montecucco C., Montecucco A. (2005)
    A new monoclonal antibody against DNA ligase I is a suitable marker of cell proliferation in cultured cell and tissue section samples.
    in European journal of histochemistry (Testo stamp.)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Vitolo B., Lidonnici M.R., Montecucco C., Montecucco A. (literal)
Pagina inizio
  • 349 (literal)
Pagina fine
  • 354 (literal)
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  • 49 (literal)
Rivista
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  • Impact Factor = 0.845 (literal)
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  • 4 (literal)
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  • Produzione e caratterizzazione di un nuovo anticorpo monoclonale diretto contro un enzima della replicazione del DNA, utilizzabile come marcatore di proliferazione cellulare. (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • L.M.R., M.A.: Istituto di Genetica Molecolare CNR, Pavia V.B., M.C.: Cattedra di Reumatologia Università di Pavia, IRCCS Policlinico San Matteo Pavia. (literal)
Titolo
  • A new monoclonal antibody against DNA ligase I is a suitable marker of cell proliferation in cultured cell and tissue section samples. (literal)
Abstract
  • The extensive characterization of the replicative human DNA ligase I (LigI) undertaken in the last decade demonstrated that the level of this protein strongly correlates with the rate of cell proliferation. This may allow to expand the repertoire of clinical biomarkers for the analysis of cell proliferation. We have produced a new monoclonal antibody (5H5) against LigI and exploited it as cell proliferation marker in Western blotting and immunofluorescence as well as in immunohistochemistry on paraffin tissue sections. The Western blot analysis showed that the LigI level detected by 5H5 antibody is high in all proliferating cells. On the contrary the protein is down regulated in resting human fibroblast and peripheral blood lymphocytes. Immunofluorescence analysis on cultured HeLa cells showed that 5H5 antibody labels all proliferating cells and displays the same staining pattern of BrdU in S-phase nuclei. Finally the analysis of serial sections of inflamed tonsils and NHL lymph nodes (either frozen or paraffin embedded) demonstrated that 5H5 marks the same population of cells as the Ki-67 antibody. Our results demonstrate that 5H5 antibody is a valuable tool for labeling proliferating cells that can be conveniently used in Western blotting, immunocytochemistry and immunohistochemistry. (literal)
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