Comparative proteomic analysis of four Bacillus clausii strains: proteomic expression signature distinguishes protein profile of the strains (Articolo in rivista)

Type
Label
  • Comparative proteomic analysis of four Bacillus clausii strains: proteomic expression signature distinguishes protein profile of the strains (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • Lippolis R., Gnoni A., Abbrescia A., Panelli D., Maiorano S., Paternoster M.S., Sardanelli A.M., Papa S., Gaballo A. (2011)
    Comparative proteomic analysis of four Bacillus clausii strains: proteomic expression signature distinguishes protein profile of the strains
    in Journal of proteomics (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Lippolis R., Gnoni A., Abbrescia A., Panelli D., Maiorano S., Paternoster M.S., Sardanelli A.M., Papa S., Gaballo A. (literal)
Pagina inizio
  • 2846 (literal)
Pagina fine
  • 2855 (literal)
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  • 74 (literal)
Rivista
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  • 15 (literal)
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  • Institute of Biomembranes and Bioenergetics, Consiglio Nazionale delle Ricerche, (CNR) Bari ItalyDepartment of Medical Biochemistry, Biology and Physics, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy (literal)
Titolo
  • Comparative proteomic analysis of four Bacillus clausii strains: proteomic expression signature distinguishes protein profile of the strains (literal)
Abstract
  • A comparative proteomic approach, using two dimensional gel electrophoresis and mass spectrometry, has been developed to compare and elucidate the differences among the cellular proteomes of four closely related isogenic O/C, SIN, N/R and T, B. clausii strains during both exponential and stationary phases of growth. Image analysis of the electropherograms reveals a high degree of concordance among the four proteomes, some proteins result, however, differently expressed. The proteins spots exhibiting high different expression level were identified, by mass-spectrometry analysis, as alcohol dehydrogenase (ADHA, EC1.2.1.3; ABC0046 isoform) aldehyde dehydrogenase (DHAS, EC 1.2.1.3; ABC0047 isoform) and flagellin-protein of B. clausii KSM-k16. The different expression levels of the two dehydrogenases were confirmed by quantitative RT- PCR and dehydrogenases enzymatic activity. The different patterns of protein expression can be considered as cell proteome signatures of the different strains. (literal)
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