Autofluorescence spectrofluorometry of central nervous system (CNS) neuromediators. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Autofluorescence spectrofluorometry of central nervous system (CNS) neuromediators. (Articolo in rivista) (literal)

Anno

• 2004-01-01T00:00:00+01:00 (literal)

Alternative label

• Crespi F, Croce AC, Fiorani S, Masala B, Heidbreder C, Bottiroli G. (2004)
  Autofluorescence spectrofluorometry of central nervous system (CNS) neuromediators.
  in Lasers in surgery and medicine (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Crespi F, Croce AC, Fiorani S, Masala B, Heidbreder C, Bottiroli G. (literal)

Pagina inizio

• 39 (literal)

Pagina fine

• 47 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 34 (literal)

Rivista

• Lasers in surgery and medicine (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• Autofluorescence spectrofluorometry of central nervous system (CNS) neuromediators. (literal)

Abstract

• BACKGROUND AND OBJECTIVES: Changes in the neurotransmitter 5-hydroxytriptamine (5-HT) are related to psychiatric diseases such as depression and anxiety. In this study, 5-HT autofluorescence properties were investigated in solution and in biological tissues. STUDY DESIGN/MATERIALS AND METHODS: Spectrofluorometric characterization was performed on ex vivo samples (tissue sections, homogenates) of the 5-HT-rich brain region hippocampus from rats untreated or treated to affect endogenous 5-HT levels; in vivo, with a 50 solidus in circle optic fiber probe positioned via stereotaxis. RESULTS: 5-HT exhibited minor excitation and emission bands at wavelengths longer than the well known excitation and emission bands in the UV region, 250-320 nm. Spectrofluorometric measurements under 366 nm excitation on homogenates supplied with 5-HT or belonging to treated rats revealed spectral alterations attributable to changes in the amount of 5-HT. Ex vivo and in vivo autofluorescence data were consistent with those obtained by conventional voltammetry. CONCLUSIONS: Autofluorescence spectroscopy potential is confirmed as a suitable technique for the direct measurement of neurotransmitters. Copyright 2004 Wiley-Liss, Inc. (literal)

Prodotto di

• Institute of molecular genetics (IGM) (Istituto)

Autore CNR

• GIOVANNI BOTTIROLI (Persona)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)

Autore CNR di

• GIOVANNI BOTTIROLI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Lasers in surgery and medicine (Rivista)