Bacterial Overexpression, Purification, and Reconstitution of the Carnitine/Acylcarnitine Carrier from Rat Liver Mitochondria (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Bacterial Overexpression, Purification, and Reconstitution of the Carnitine/Acylcarnitine Carrier from Rat Liver Mitochondria (Articolo in rivista) (literal)

Anno

• 1998-01-01T00:00:00+01:00 (literal)

Alternative label

• Cesare Indiveri, Vito Iacobazzi, Nicola Giangregorio, and Ferdinando Palmieri (1998)
  Bacterial Overexpression, Purification, and Reconstitution of the Carnitine/Acylcarnitine Carrier from Rat Liver Mitochondria
  in Biochemical and biophysical research communications (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Cesare Indiveri, Vito Iacobazzi, Nicola Giangregorio, and Ferdinando Palmieri (literal)

Pagina inizio

• 589 (literal)

Pagina fine

• 594 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 249 (literal)

Rivista

• Biochemical and biophysical research communications (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 6 (literal)

Note

• ISI Web of Science (WOS) (literal)

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• Department of Pharmaco-Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari, Via E. Orabona 4, 70125 Bari, Italy; and CNR Unit for the Study of Mitochondria and Bioenergetics, Bari, Italy (literal)

Titolo

• Bacterial Overexpression, Purification, and Reconstitution of the Carnitine/Acylcarnitine Carrier from Rat Liver Mitochondria (literal)

Abstract

• The carnitine/acylcarnitine carrier from rat liver mitochondria was overexpressed inEscherichia coli.The expressed protein, recovered as inclusion bodies, was solubilized with sarkosyl and purified by Sephadex G-200 and celite chromatography. A yield of 15 mg of purified transport protein per liter of cell culture was obtained. Upon reconstitution into liposomes, the purified carrier catalyzed a [3H]carnitine/carnitine exchange inhibited by maleimides, mercurials, and sulfobetaines. Carnitine esters of various lengths were also transported. The Kmfor carnitine uptake was 0.47 ± 0.11 mM, the Vmaxof the exchange was 0.78 ± 0.24 mmol/min per gram of protein, and the Kifor octanoylcarnitine was 13.5 ± 4.3 ?M. The transport properties of the recombinant carrier were virtually identical to those of the native transporter. These studies represent the first overexpression of the functionally active mitochondrial carnitine/acylcarnitine carrier, thus enabling structure/function analysis of this protein by site-directed mutagenesis. (literal)

Prodotto di

• Istituto di biomembrane e bioenergetica (IBBE) (Istituto)
• Trasportatori mitocondriali: struttura e meccanismi funzionali (SV.P14.007.001) (Modulo)

Autore CNR

• vito iacobazzi (Unità di personale esterno)
• CESARE INDIVERI (Persona)
• FERDINANDO PALMIERI (Unità di personale esterno)
• NICOLA GIANGREGORIO (Persona)

Incoming links:

Prodotto

• Istituto di biomembrane e bioenergetica (IBBE) (Istituto)
• Trasportatori mitocondriali: struttura e meccanismi funzionali (SV.P14.007.001) (Modulo)

Autore CNR di

• NICOLA GIANGREGORIO (Persona)
• CESARE INDIVERI (Persona)
• FERDINANDO PALMIERI (Unità di personale esterno)
• vito iacobazzi (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biochemical and biophysical research communications (Rivista)