A placental growth factor variant unable to recognize vascular endothelial growth factor (VEGF) receptor-1 inhibits VEGF-dependent tumor angiogenesis via heterodimerization (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• A placental growth factor variant unable to recognize vascular endothelial growth factor (VEGF) receptor-1 inhibits VEGF-dependent tumor angiogenesis via heterodimerization (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1158/0008-5472.CAN-09-2609 (literal)

Alternative label

• Tarallo V.; Vesci L.; Capasso O.; Esposito M.T.; Riccioni T.; Pastore L.; Orlandi A.; Pisano C. and De Falco S. (2010)
  A placental growth factor variant unable to recognize vascular endothelial growth factor (VEGF) receptor-1 inhibits VEGF-dependent tumor angiogenesis via heterodimerization
  in Cancer research (Chic. Ill.); American Association For Cancer Research, Philadelphia (Stati Uniti d'America)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Tarallo V.; Vesci L.; Capasso O.; Esposito M.T.; Riccioni T.; Pastore L.; Orlandi A.; Pisano C. and De Falco S. (literal)

Pagina inizio

• 1804 (literal)

Pagina fine

• 1813 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://cancerres.aacrjournals.org/content/70/5/1804.long (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 70 (literal)

Rivista

• Cancer research (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• PMID: 20145150 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 5 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Angiogenesis Lab and Stem Cell Fate Lab, Institute of Genetics and Biophysics \"Adriano Buzzati-Traverso,\" CNR, Napoli, Italy Centro di Ricerca per l'Ingegneria Genetica-Biotecnologie Avanzate; Napoli, Italy Department of Biochemistry and Medical Biotechnology, University of Naples \"Federico II,\" Naples, Italy Research and Development Oncology Area, Sigma-Tau S.p.A., Industrie Farmaceutiche Riunite; Pomezia (Roma), Italy Anatomic Pathology Institute, Department of Biopathology and Image Diagnostics, Tor Vergata University, Roma, Italy (literal)

Titolo

• A placental growth factor variant unable to recognize vascular endothelial growth factor (VEGF) receptor-1 inhibits VEGF-dependent tumor angiogenesis via heterodimerization (literal)

Abstract

• Angiogenesis is one of the crucial events for cancer development and growth. Two members of the vascular endothelial growth factor (VEGF) family, VEGF-A and placental growth factor (PlGF), which are able to heterodimerize if coexpressed in the same cell, are both required for pathologic angiogenesis. We have generated a PlGF1 variant, named PlGF1-DE in which the residues Asp72 and Glu73 were substituted with Ala, which is unable to bind and activate VEGF receptor-1 but is still able to heterodimerize with VEGF. Here, we show that overexpression in tumor cells by adenoviral delivery or stable transfection of PlGF1-DE variant significantly reduces the production of VEGF homodimer via heterodimerization, determining a strong inhibition of xenograft tumor growth and neoangiogenesis, as well as significant reduction of vessel lumen and stabilization, and monocyte-macrophage infiltration. Conversely, the overexpression of PlGF1wt, also reducing the VEGF homodimer production comparably with PlGF1-DE variant through the generation of VEGF/PlGF heterodimer, does not inhibit tumor growth and vessel density compared with controls but induces increase of vessel lumen, vessel stabilization, and monocyte-macrophage infiltration. The property of PlGF and VEGF-A to generate heterodimer represents a successful strategy to inhibit VEGF-dependent angiogenesis. The PlGF1-DE variant, and not PlGF1wt as previously reported, acts as a \"dominant negative\" of VEGF and is a new candidate for antiangiogenic gene therapy in cancer treatment. (literal)

Editore

• American association for cancer research (Editore)

Prodotto di

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Identificazione di regolatori del differenziamento, della motilità e dell'apoptosi delle cellule staminali (SV.P15.008.001) (Modulo)

Autore CNR

• Valeria Tarallo (Persona)
• SANDRO DE FALCO (Unità di personale interno)
• Onofrio Capasso (Persona)

Insieme di parole chiave

• Parole chiave di "A placental growth factor variant unable to recognize vascular endothelial growth factor (VEGF) receptor-1 inhibits VEGF-dependent tumor angiogenesis via heterodimerization" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Identificazione di regolatori del differenziamento, della motilità e dell'apoptosi delle cellule staminali (SV.P15.008.001) (Modulo)

Autore CNR di

• SANDRO DE FALCO (Unità di personale interno)
• Valeria Tarallo (Persona)
• Onofrio Capasso (Persona)

Editore di

• American association for cancer research (Editore)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Cancer research (Rivista)

Insieme di parole chiave di

• Parole chiave di "A placental growth factor variant unable to recognize vascular endothelial growth factor (VEGF) receptor-1 inhibits VEGF-dependent tumor angiogenesis via heterodimerization" (Insieme di parole chiave)