Flt-1 expression influences apoptotic susceptibility of vascular smooth muscle cells through NF-kappaB / IAP-1 pathway (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Flt-1 expression influences apoptotic susceptibility of vascular smooth muscle cells through NF-kappaB / IAP-1 pathway (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1093/cvr/cvp288 (literal)

Alternative label

• Orlandi A, Ferlosio A, Arcuri G, Scioli MG, De Falco S, Spagnoli LG. (2010)
  Flt-1 expression influences apoptotic susceptibility of vascular smooth muscle cells through NF-kappaB / IAP-1 pathway
  in Cardiovascular research; Oxford University Press, Oxford (Regno Unito)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Orlandi A, Ferlosio A, Arcuri G, Scioli MG, De Falco S, Spagnoli LG. (literal)

Pagina inizio

• 214 (literal)

Pagina fine

• 223 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://cardiovascres.oxfordjournals.org/content/85/1/214.long (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 85 (literal)

Rivista

• Cardiovascular research (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• PMID: 19720604 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Univ Roma Tor Vergata, Dept Biopathol & Image Diagnost, I-00133 Rome, Italy 2. CNR, Inst Genet & Biophys, I-80131 Naples, Italy (literal)

Titolo

• Flt-1 expression influences apoptotic susceptibility of vascular smooth muscle cells through NF-kappaB / IAP-1 pathway (literal)

Abstract

• Aims Flt-1 is an fms-like tyrosine kinase receptor which binds to vascular endothelial growth factor (VEGF) and placental growth factor (PlGF). Ligand activation and blocking of flt-1 influence several vascular smooth muscle cell (SMC) functions, including apoptotic susceptibility. However, downstream signal transduction pathways by which flt-1 regulates SMC apoptosis have still to be investigated. Methods and results Flt-1 expression and apoptosis in Wistar rat aortic intimal cells 15 days after ballooning were studied by immunohistochemistry, cytometry, cell sorting, western blotting, and PCR. Anti-flt1 blocking antibody effects were compared with those of anti-PlGF and anti-VEGF antibodies. Rat aortic intimal cells 15 days after injury exhibited increased flt-1 protein and mRNA and lower smooth muscle markers compared with normal media SMCs. Immunoreactivity for flt-1 protein was also observed in apoptotic intimal cells. Anti-flt-1 (EC(50) = 16.5 ng/mL) and anti-PlGF (EC(50) = 20.5 ng/mL) antibodies added to intimal cultures reduced serum-deprived apoptosis but not serum-and PDGF-BB-induced proliferation; the anti-VEGF antibody was ineffective. Sorted flt-1(+) cells were more clonogenic than flt-1 and whole intimal SMC populations. Increased nuclear factor-kappaB (NF-kappa B) and inhibitor of apoptosis protein-1 (IAP-1) and reduced bax levels associated with the anti-flt-1-induced increase of intimal SMC survival; the latter was prevented by NF-kappa B activity inhibitor and IAP-1 interfering RNA (RNAi). Blocking of NF-kappa B activity reduced IAP-1 expression and prevented IAP-1 RNAi effects. Increased flt-1 immunoreaction was also documented in human atheromatous lesions. Conclusion Our results show that anti-flt-1 blocking reduces apoptosis through NF-kappa B and the downstream IAP-1 pathway. The close link between flt-1, PlGF, and apoptotic susceptibility of intimal SMCs suggests new potential strategies aimed at influencing post-injury arterial remodelling. (literal)

Editore

• Oxford University Press (Editore)

Prodotto di

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Identificazione di regolatori del differenziamento, della motilità e dell'apoptosi delle cellule staminali (SV.P15.008.001) (Modulo)

Autore CNR

• SANDRO DE FALCO (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Flt-1 expression influences apoptotic susceptibility of vascular smooth muscle cells through NF-kappaB / IAP-1 pathway" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Identificazione di regolatori del differenziamento, della motilità e dell'apoptosi delle cellule staminali (SV.P15.008.001) (Modulo)

Autore CNR di

• SANDRO DE FALCO (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Cardiovascular research (Rivista)

Editore di

• Oxford University Press (Editore)

Insieme di parole chiave di

• Parole chiave di "Flt-1 expression influences apoptotic susceptibility of vascular smooth muscle cells through NF-kappaB / IAP-1 pathway" (Insieme di parole chiave)