Growth conditions differentially affect the constitutive expression of primary response genes in cultured cerebellar granule cells. (Articolo in rivista)

Type
Label
  • Growth conditions differentially affect the constitutive expression of primary response genes in cultured cerebellar granule cells. (Articolo in rivista) (literal)
Anno
  • 1995-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/BF01694544 (literal)
Alternative label
  • 1, 2 Copani A., 1, 2 Bruno V., 3 Dell'Albani P., 1,2 Battaglia G., 3 Barresi V., 3 Caruso A., 1, 4 Nicoletti F., 3 Condorelli D.F. (1995)
    Growth conditions differentially affect the constitutive expression of primary response genes in cultured cerebellar granule cells.
    in Neurochemical research
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • 1, 2 Copani A., 1, 2 Bruno V., 3 Dell'Albani P., 1,2 Battaglia G., 3 Barresi V., 3 Caruso A., 1, 4 Nicoletti F., 3 Condorelli D.F. (literal)
Pagina inizio
  • 611 (literal)
Pagina fine
  • 616 (literal)
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  • 20 (literal)
Rivista
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  • 6 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 5 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • Google Scholar (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Institutes of (1) Pharmacology and (2) Neurological Sciences and (3) Biochemistry, School of Medicine , University of Catania ; (4) Department of Experimental Medicine and Biochemical Sciences, Pharmacology Section,University of Perugia, Italy (literal)
Titolo
  • Growth conditions differentially affect the constitutive expression of primary response genes in cultured cerebellar granule cells. (literal)
Abstract
  • Cultured cerebellar granule cells underwent apoptotic degeneration when grown in medium containing 10 instead of 25 mM K+. Knowing that apoptosis is associated with changes in the expression of primary response genes, we have measured c-fos, zif/268, and c-jun mRNA levels during maturation of cultured granule cells grown in 10 or 25 mM K+. The constitutive expression of c-fos and zif/268 was differentially regulated by extracellular K+ concentration at 5 days of maturation in vitro (DIV), when cells grown under suboptimal conditions (i.e. in 10 mM K+) are committed to degenerate. At this stage, c-fos mRNA levels were detectable only in cultures grown in 25 mM K+, whereas zif/268 mRNA levels were dramatically elevated in cultures grown in 10 mM K+. This provides one of the few conditions in which c-fos and zif/268 are differentially regulated in nerve cells. Substantial changes in c-jun, or beta-actin mRNA levels were detectable only at 7 DIV, when the percentage of apoptotic cells had already reached a plateau in cultures grown in 10 mM K+. We speculate that changes in the expression of zif/268 are important in the gene program associated with the induction of apoptosis by trophic deprivation in cultured neurons. (literal)
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