Detrimental effects of Bartonella henselae are counteracted by L-arginine and nitric oxide in human endothelial progenitor cells (Articolo in rivista)

Type
Label
  • Detrimental effects of Bartonella henselae are counteracted by L-arginine and nitric oxide in human endothelial progenitor cells (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1073/pnas.0803602105 (literal)
Alternative label
  • Salvatore P.; Casamassimi A.; Sommese L.; Fiorito C.; Ciccodicola A.; Rossiello R.; Avallone B.; Grimaldi V.; Costa V.; Rienzo M.; Colicchio R.; Williams-Ignarro S.; Pagliarulo C.; Prudente M.E.; Abbondanza C.; Lamberti F.;Baroni A.; Buommino E.; Farzati B.; Tufano M.A.; Ignarro L.J. and Napoli C. (2008)
    Detrimental effects of Bartonella henselae are counteracted by L-arginine and nitric oxide in human endothelial progenitor cells
    in Proceedings of the National Academy of Sciences of the United States of America
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Salvatore P.; Casamassimi A.; Sommese L.; Fiorito C.; Ciccodicola A.; Rossiello R.; Avallone B.; Grimaldi V.; Costa V.; Rienzo M.; Colicchio R.; Williams-Ignarro S.; Pagliarulo C.; Prudente M.E.; Abbondanza C.; Lamberti F.;Baroni A.; Buommino E.; Farzati B.; Tufano M.A.; Ignarro L.J. and Napoli C. (literal)
Pagina inizio
  • 9427 (literal)
Pagina fine
  • 9432 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 105 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 6 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 27 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Univ Calif Los Angeles, David Geffen Sch Med, Dept Mol Pharmacol, Los Angeles, CA 90095 USA Univ Naples Federico 2, Dept Cellular & Mol Biol & Pathol Luigi Califano, I-80131 Naples, Italy Univ Naples Federico 2, Sch Biotechnol Sci, I-80131 Naples, Italy Univ Naples 2, Dept Gen Pathol & Excellence Res, Ctr Cardiovasc Dis, Sch Med 1, I-80138 Naples, Italy Univ Naples 2, Microbiol Sect, Dept Expt Med, I-80138 Naples, Italy Univ Naples 2, Dept Human Pathol, I-80138 Naples, Italy Univ Naples 2, Dept Dermatol, I-80138 Naples, Italy Ist Ricovero & Cura Carattere Sci Multimed, I-20099 Milan, Italy Consiglio Nazl Ric Inst Genet & Biophys A Buzzati, I-80131 Naples, Italy Univ Naples Federico 2, Dept Biol Sci, Sect Genet & Mol Biol, I-80134 Naples, Italy Ist Ricovero & Cura Carattere Sci, SDN Res Fdn, I-80143 Naples, Italy Univ Sannio, Dept Biol & Environm Sci, I-82110 Benevento, Italy Univ Calif Los Angeles, David Geffen Sch Med, Div Anesthesiol, Los Angeles, CA 90095 USA (literal)
Titolo
  • Detrimental effects of Bartonella henselae are counteracted by L-arginine and nitric oxide in human endothelial progenitor cells (literal)
Abstract
  • The recruitment of circulating endothelial progenitor cells (EPCs) might have a beneficial effect on the clinical course of several diseases. Endothelial damage and detachment of endothelial cells are known to occur in infection, tissue ischemia, and sepsis. These detrimental effects in EPCs are unknown. Here we elucidated whether human EPCs internalize Bartonella henselae constituting a circulating niche of the pathogen. B. henselae invades EPCs as shown by gentamicin protection assays and transmission electron microscopy (TEM). Dil-Ac-LDL/lectin double immunostaining and fluorescence-activated cell sorting (FACS) analysis of EPCs revealed EPC bioactivity after infection with B. henselae. Nitric oxide (NO) and its precursor L-arginine (L-arg) exert a plethora of beneficial effects on vascular function and modulation of immune response. Therefore, we tested also the hypothesis that L-arg (1-30 mM) would affect the infection of B. henselae or tumor necrosis factor (TNF) in EPCs. Our data provide evidence that L-arg counteracts detrimental effects induced by TNF or Bartonella infections via NO (confirmed by DETA-NO and L-NMMA experiments) and by modulation of p38 kinase phosphorylation. Microarray analysis indicated several genes involved in immune response were differentially expressed in Bartonella-infected EPCs, whereas these genes returned in steady state when cells were exposed to sustained doses Of L-arg. This mechanism may have broad therapeutic applications in tissue ischemia, angiogenesis, immune response, and sepsis. (literal)
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