Cripto recruits Furin and PACE4 and controls Nodal trafficking during proteolytic maturation (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Cripto recruits Furin and PACE4 and controls Nodal trafficking during proteolytic maturation (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1038/emboj.2008.174 (literal)

Alternative label

• Blanchet M.H.; Le Good J.A.; Mesnard D.; Oorschot V.; Baflast S.; Minchiotti G.; Klumperman J.; Constam D.B. (2008)
  Cripto recruits Furin and PACE4 and controls Nodal trafficking during proteolytic maturation
  in EMBO journal (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Blanchet M.H.; Le Good J.A.; Mesnard D.; Oorschot V.; Baflast S.; Minchiotti G.; Klumperman J.; Constam D.B. (literal)

Pagina inizio

• 2580 (literal)

Pagina fine

• 2591 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2567404/?tool=pubmed (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 27 (literal)

Rivista

• EMBO journal (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 19 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Swiss Inst Expt Canc Res ISREC, EPFL, CH-1066 Epalinges, Switzerland Cell Microscopy Ctr, Dept Cell Biol, Utrecht, Netherlands Inst Genet & Biophys A Buzzati Traverso, Naples, Italy (literal)

Titolo

• Cripto recruits Furin and PACE4 and controls Nodal trafficking during proteolytic maturation (literal)

Abstract

• The glycosylphosphatidylinositol (GPI)-anchored proteoglycan Cripto binds Nodal and its type I receptor Alk4 to activate Smad2,3 transcription factors, but a role during Nodal precursor processing has not been described. We show that Cripto also binds the proprotein convertases Furin and PACE4 and localizes Nodal processing at the cell surface. When coexpressed as in early embryonic cells, Cripto and uncleaved Nodal already associated during secretion, and a Cripto-interacting region in the Nodal propeptide potentiated the effect of proteolytic maturation on Nodal signalling. Disruption of the trans-Golgi network (TGN) by brefeldin A blocked secretion, but export of Cripto and Nodal to the cell surface was not inhibited, indicating that Nodal is exposed to extracellular convertases before entering the TGN/endosomal system. Density fractionation and antibody uptake experiments showed that Cripto guides the Nodal precursor in detergent-resistant membranes to endocytic microdomains marked by GFP-Flotillin. We conclude that Nodal processing and endocytosis are coupled in signal-receiving cells. (literal)

Prodotto di

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Identificazione di regolatori del differenziamento, della motilità e dell'apoptosi delle cellule staminali (SV.P15.008.001) (Modulo)

Autore CNR

• GABRIELLA MINCHIOTTI (Persona)

Insieme di parole chiave

• Keywords of "Cripto recruits Furin and PACE4 and controls Nodal trafficking during proteolytic maturation" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Identificazione di regolatori del differenziamento, della motilità e dell'apoptosi delle cellule staminali (SV.P15.008.001) (Modulo)

Autore CNR di

• GABRIELLA MINCHIOTTI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• EMBO journal (Rivista)

Insieme di parole chiave di

• Keywords of "Cripto recruits Furin and PACE4 and controls Nodal trafficking during proteolytic maturation" (Insieme di parole chiave)