A new isoluminol reagent for chemiluminescence labeling of proteins (Articolo in rivista)

Type
Label
  • A new isoluminol reagent for chemiluminescence labeling of proteins (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.tetlet.2013.06.036 (literal)
Alternative label
  • A. Palmioli, M. Crisma, C. Peggion, P.N. Brusasca, D. Zanin, A. Dal Corso, P. Ingallinella, F. Peri (2013)
    A new isoluminol reagent for chemiluminescence labeling of proteins
    in Tetrahedron letters
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • A. Palmioli, M. Crisma, C. Peggion, P.N. Brusasca, D. Zanin, A. Dal Corso, P. Ingallinella, F. Peri (literal)
Pagina inizio
  • 4446 (literal)
Pagina fine
  • 4450 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • IF 2012: 2.397 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 54 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza, 2, 20126 Milano, Italy; Institute of Biomolecular Chemistry, Padova Unit, CNR, Department of Chemistry, University of Padova, 35131 Padova, Italy; DiaSorin Research Center, via Lepetit 34, 21040 Gerenzano (VA), Italy; Product Development, DiaSorin SpA, via Crescentino, 13040 Saluggia (VC), Italy (literal)
Titolo
  • A new isoluminol reagent for chemiluminescence labeling of proteins (literal)
Abstract
  • We report the synthesis and the characterization of N-(4-aminobutyl)-N-ethyl-isoluminol (ABEI) macrocyclic lactone, an activated ester with an unusual macrocyclic structure, and its use for efficient ABEI conjugation to proteins. Compared to the equivalent reagent normally used for chemiluminescence protein labeling, the ABEI macrocyclic lactone displays improved chemical properties, including stability and reactivity. We show the simple synthesis and the use of ABEI macrocyclic lactone for efficient chemiluminescence labeling of monoclonal antibody mixtures currently used in clinical immunodiagnostic assays for the detection of the HIV p24 antigen in patients. (literal)
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