Plgf-/-eNos-/- mice show defective angiogenesis associated with increased oxidative stress in response to tissue ischemia (Articolo in rivista)

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  • Plgf-/-eNos-/- mice show defective angiogenesis associated with increased oxidative stress in response to tissue ischemia (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1096/fj.05-4481fje (literal)
Alternative label
  • Gigante B.; Morlino G.; Gentile M.T.; Persico M.G.; De Falco S. (2006)
    Plgf-/-eNos-/- mice show defective angiogenesis associated with increased oxidative stress in response to tissue ischemia
    in The FASEB journal; Federation Of American Societies Of Experimental Biology, Bethesda (Stati Uniti d'America)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Gigante B.; Morlino G.; Gentile M.T.; Persico M.G.; De Falco S. (literal)
Pagina inizio
  • 970 (literal)
Pagina fine
  • 972 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.fasebj.org/content/20/7/970.long (literal)
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  • 20 (literal)
Rivista
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  • 7 (literal)
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  • ISI Web of Science (WOS) (literal)
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  • CNR, Inst Genet & Biophys Adriano Buzzati Traverso, I-80131 Naples, Italy; Ist Ric & Cura Caratere Sci Neuromed, Pozzilli, IS, Italy. (literal)
Titolo
  • Plgf-/-eNos-/- mice show defective angiogenesis associated with increased oxidative stress in response to tissue ischemia (literal)
Abstract
  • Neo-angiogenesis is a complex phenomenon modulated by the concerted action of several molecular factors. We have generated a congenic line of knockout mice carrying null mutations of both placental growth factor (PlGF) and endothelial nitric oxide synthase (eNOS), two genes that play a pivotal role in the regulation of pathological angiogenesis. In the present study, we describe the phenotype of this new experimental animal model after surgically induced hind-limb ischemia. Plgf–/–, eNos–/–, Plgf–/– eNos–/–, and wild-type C57BL/6J mice were studied. Plgf–/– eNos–/– mice showed the most severe phenotype: self-amputation, and death occurred in up to 47% of the animals studied; in ischemic legs, capillary density was severely reduced; macrophage infiltration and oxidative stress increased as compared to the other groups of animals. These changes were associated with an up-regulation of both inducible NOS (iNOS) expression and vascular endothelial growth factor (VEGF) protein levels in ischemic limbs, and to an increased extent of protein nitration. Our results demonstrate that the deletion of these two genes, Plgf, which acts in synergism with VEGF, and eNos, a downstream mediator of VEGF, determines a significant change in the vascular response to an ischemic stimulus and that oxidative stress within the ischemic tissue represents a crucial factor to maintain tissue homeostasis.—Gigante, B., Morlino, G., Gentile, M. T., Persico, M. G., De Falco, S. Plgf–/–eNos–/– mice show defective angiogenesis associated with increased oxidative stress in response to tissue ischemia. (literal)
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