Forebrain and midbrain development requires epiblast-restricted Otx2 translational control mediated by its 3' UTR (Articolo in rivista)

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Label
  • Forebrain and midbrain development requires epiblast-restricted Otx2 translational control mediated by its 3' UTR (Articolo in rivista) (literal)
Anno
  • 2001-01-01T00:00:00+01:00 (literal)
Alternative label
  • Boyl PP, Signore M, Acampora D, Martinez-Barbera JP, Ilengo C, Annino A, Corte G, Simeone A. (2001)
    Forebrain and midbrain development requires epiblast-restricted Otx2 translational control mediated by its 3' UTR
    in Development (Camb.)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Boyl PP, Signore M, Acampora D, Martinez-Barbera JP, Ilengo C, Annino A, Corte G, Simeone A. (literal)
Pagina inizio
  • 2989 (literal)
Pagina fine
  • 3000 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 128 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 MRC Centre for Developmental Neurobiology, King’s College London, Guy’s Campus, New Hunt’s House, London SE1 1UL, UK 2 International Institute of Genetics and Biophysics, CNR, Via G. Marconi 12, 80125, Naples, Italy 3 IST-National Institute for Cancer Research, Università di Genova, Largo Benzi, 16132 Genova, Italy 4 Dipartimento di Oncologia Clinica e Sperimentale, Università di Genova, Largo Benzi, 16132 Genova, Italy (literal)
Titolo
  • Forebrain and midbrain development requires epiblast-restricted Otx2 translational control mediated by its 3' UTR (literal)
Abstract
  • Otx genes play an important role in brain development. Previous mouse models suggested that the untranslated regions (UTRs) of Otx2 mRNA may contain regulatory element(s) required for its post-transcriptional control in epiblast and neuroectoderm. In order to study this, we have perturbed the 3' UTR of Otx2 by inserting a small fragment of DNA from the phage. Otx2 mutants exhibited proper gastrulation and normal patterning of the early anterior neural plate, but from 8.5 days post coitum they developed severe forebrain and midbrain abnormalities. OTX2 protein levels in Otx2 mutants were heavily reduced in the epiblast, axial mesendoderm and anterior neuroectoderm but not in the visceral endoderm. At the molecular level, we found out that the ability of the Otx2 mRNA to form efficient polyribosome complexes was impaired. Sequence analysis of the Otx2-3' UTR revealed a 140 bp long element that is present only in vertebrate Otx2 genes and conserved in identity by over 80%. Our data provide experimental evidence that murine brain development requires accurate translational control of Otx2 mRNA in epiblast and neuronal progenitor cells. This leads us to hypothesise that this control might have important evolutionary implications. (literal)
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