http://www.cnr.it/ontology/cnr/individuo/prodotto/ID26377
The expression in tobacco plants of Aedes aegypti Trypsin Modulating Oostatic Factor (Aea-TMOF) alters growth and development of the tobacco budworm, Heliothis virescens (Articolo in rivista)
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- Label
- The expression in tobacco plants of Aedes aegypti Trypsin Modulating Oostatic Factor (Aea-TMOF) alters growth and development of the tobacco budworm, Heliothis virescens (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
- Alternative label
Tortiglione C., Fanti P., Pennacchio F., Malva C., Breuer M., De Loof A., Monti L.M., Tremblay E., Rao R. (2002)
The expression in tobacco plants of Aedes aegypti Trypsin Modulating Oostatic Factor (Aea-TMOF) alters growth and development of the tobacco budworm, Heliothis virescens
in Molecular breeding
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Tortiglione C., Fanti P., Pennacchio F., Malva C., Breuer M., De Loof A., Monti L.M., Tremblay E., Rao R. (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
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- ISI Web of Science (WOS) (literal)
- Titolo
- The expression in tobacco plants of Aedes aegypti Trypsin Modulating Oostatic Factor (Aea-TMOF) alters growth and development of the tobacco budworm, Heliothis virescens (literal)
- Abstract
- The production and characterisation of transgenic tobacco plants
expressing a precursor of a regulatory peptide from Aedes aegypti
(Trypsin Modulating and Oostatic Factor, Aea-TMOF) which interferes
with the development of tobacco budworm larvae is described.
Tobacco plants were transformed with a synthetic gene containing 6
TMOF units spaced by dibasic residues, Arg-Arg, as potential
post-translational cleavage sites. Peptide extracts from transgenic
plants had TMOF activity and inhibited in vitro the biosynthesis of
serine proteases. This activity was consistently present in T1 plants
and absent in control plants. Tobacco budworm larvae, fed with
transgenic leaves showed a reduced growth rate compared to those
fed with control plants. The low rather than acute toxicity of this low
impact gene is discussed in the context of alternative integrated pest
management strategies.
(literal)
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