Cell Uptake Enhancement of Folate Targeted Polymer Coated Magnetic Nanoparticles (Articolo in rivista)

Type
Label
  • Cell Uptake Enhancement of Folate Targeted Polymer Coated Magnetic Nanoparticles (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1166/jbn.2013.1606 (literal)
Alternative label
  • Licciardi M, Scialabba C, Cavallaro G, Sangregorio C, Fantechi E, Giammona G. (2013)
    Cell Uptake Enhancement of Folate Targeted Polymer Coated Magnetic Nanoparticles
    in Journal of biomedical nanotechnology (Print); American scientific publishers, Valencia [Ca] (Stati Uniti d'America)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Licciardi M, Scialabba C, Cavallaro G, Sangregorio C, Fantechi E, Giammona G. (literal)
Pagina inizio
  • 949 (literal)
Pagina fine
  • 964 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 9 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 5 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 6 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 ] Univ Palermo, Lab Biocompatible Polymers, Dept Sci & Tecnol Mol & Biomol, I-90123 Palermo, Italy 2 ] ISTM CNR, I-20133 Milan, Italy 3 ] Univ Florence, INSTM, I-50019 Florence, Italy 4 ] Univ Florence, Dept Chem U Schiff, I-50019 Florence, Italy 5 ] IBF CNR, I-90143 Palermo, Italy (literal)
Titolo
  • Cell Uptake Enhancement of Folate Targeted Polymer Coated Magnetic Nanoparticles (literal)
Abstract
  • Dual targeted drug delivery systems represent a potential platform for developing efficient vector to tumor sites. In this study we evaluated a folate- and magnetic-targeted nanocarriers based on 10 nm iron oxide nanodomais coated with the properly synthesized and characterized folic acid (FA)-functionalized amphiphilic copolymer PHEA-PLA-PEG-FA. FA was chemically conjugated to one end of diamino-polyethylene glycol of 2000 Da, in order to ensure its exposition on the polymer coated magnetic nanoparticles (MNPs-FA). The prepared nanoparticles have been exhaustively characterized by different methods, including DLS, SEM, FT-IR and magnetic measurements. Magnetic nanoparticles showed dimension of about 37 nm with a narrow size distribution and a characteristic superparamagnetic behaviour. The lack of cytotoxicity of MNPs-FA and MNPs was assessed both on MCF7 cells, used as a model tumor cell line, and on 16HBE, used as normal human cell model, by evaluating cell viability using MTS assay, while the preferential internalization of MNPs-FA into tumor cells rather that into normal cells was confirmed by the quantization of internalized iron oxide. Uptake studies were also performed in the presence of a permanent magnet in order to verify the synergistic effect of magnetic field in enhancing the internalization of magnetic nanoparticles. Finally, real-time confocal microscopy experiments were carried out to further confirmed that FA ligand enhances the MNPs-FA accumulation into cancer cell cytoplasm. (literal)
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