Molecular characterisation of a cloned DNA from a tomato yellow leaf curl virus isolate form Sicily (Articolo in rivista)

Type
Label
  • Molecular characterisation of a cloned DNA from a tomato yellow leaf curl virus isolate form Sicily (Articolo in rivista) (literal)
Anno
  • 1995-01-01T00:00:00+01:00 (literal)
Alternative label
  • Crespi, S.. Noris, E., Vaira, A. M., Accotto, G. P. (1995)
    Molecular characterisation of a cloned DNA from a tomato yellow leaf curl virus isolate form Sicily
    in Phytopathologia Mediterranea (Testo stamp.)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Crespi, S.. Noris, E., Vaira, A. M., Accotto, G. P. (literal)
Pagina inizio
  • 93 (literal)
Pagina fine
  • 99 (literal)
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  • http://www.cabdirect.org/abstracts/19952311031.html (literal)
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  • 34 (literal)
Rivista
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  • 2 (literal)
Note
  • Cab abstracts (literal)
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  • CS, NE, VAM, AGP: IVV CNR Str delle Cacce 73, 10135 Turin Italy (literal)
Titolo
  • Molecular characterisation of a cloned DNA from a tomato yellow leaf curl virus isolate form Sicily (literal)
Abstract
  • A DNA molecule, 2773 nucleotides long, was isolated from tomato plants infected by tomato yellow leaf curl bigeminivirus in Sicily, Italy (TYLCV-Sc). The DNA was cloned and sequenced. Comparisons with other TYLCV isolates from Sardinia and Spain (western Mediterranean region) showed high total sequence homology (>90%), with lower homology levels (80-84%) in the intergenic regions. Total DNA homology dropped to 76% when TYLCV-Sc was compared with an isolate from Israel (TYLCV-Is). The lower homology was confirmed by the use of digoxigenin-labelled probes prepared from TYLCV-Sc and TYLCV-Is. In dot blot experiments on samples collected in different Mediterranean areas, the TYLCV-Sc probe reacted strongly with western Mediterranean isolates and moderately with those from eastern Mediterranean zones. When cloned TYLCV-Sc DNA were replicated in tobacco protoplasts, no differences were observed in viral DNA forms, compared with DNA of TYLCV-Sr (a Sardinian isolate). (literal)
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