DNA-binding activity of the C2 protein of tomato yellow leaf curl geminivirus (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• DNA-binding activity of the C2 protein of tomato yellow leaf curl geminivirus (Articolo in rivista) (literal)

Anno

• 1996-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1006/viro.1996.0157 (literal)

Alternative label

• Noris, E., Jupin, I., Accotto, G.P., Gronenborn, B. (1996)
  DNA-binding activity of the C2 protein of tomato yellow leaf curl geminivirus
  in Virology (N.Y.N.Y., Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Noris, E., Jupin, I., Accotto, G.P., Gronenborn, B. (literal)

Pagina inizio

• 607 (literal)

Pagina fine

• 612 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.sciencedirect.com/science/article/pii/S0042682296901579 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 217 (literal)

Rivista

• Virology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 5 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 2 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• NE, AGP, IVV CNR, str delle Cacce 73, 10135 Turin, Italy JI, GB, ISV, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France (literal)

Titolo

• DNA-binding activity of the C2 protein of tomato yellow leaf curl geminivirus (literal)

Abstract

• The in vitro DNA-binding activity of the C2 protein of tomato yellow leaf curl geminivirus (TYLCV) was studied following its expression in Escherichia coli as a fusion protein with an His tag N-terminal extension (His-C2). Southwestern blotting experiments demonstrated that the C2 protein is able to bind both single-stranded and double-stranded DNA probes. In electrophoretic mobility shift assays performed using purified protein and single-stranded DNA probes several shifted complexes were formed. The presence of NaCl (up to 800 mM) did not substantially affect binding profiles, demonstrating a stable interaction. His-CP appeared to bind single-stranded DNA in a sequence-nonspecific manner, with a preference for single-stranded compared to double-stranded DNA. Deletion mutants demonstrated that the central core of C2 (amino acids 33 to 104), which contains a Cys-His rich region, is sufficient for conferring binding activity. The potential significance of this DNA-binding activity with respect to possible biological functions of TYLCV C2 protein is discussed. (literal)

Autore CNR

• EMANUELA NORIS (Unità di personale interno)
• GIAN PAOLO ACCOTTO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "DNA-binding activity of the C2 protein of tomato yellow leaf curl geminivirus" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• GIAN PAOLO ACCOTTO (Unità di personale interno)
• EMANUELA NORIS (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Virology (Rivista)

Insieme di parole chiave di

• Keywords of "DNA-binding activity of the C2 protein of tomato yellow leaf curl geminivirus" (Insieme di parole chiave)