Typing of tomato yellow leaf curl viruses in Europe (Articolo in rivista)

Type
Label
  • Typing of tomato yellow leaf curl viruses in Europe (Articolo in rivista) (literal)
Anno
  • 2000-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1023/A:1008736023293 (literal)
Alternative label
  • Accotto, G.P., Navas-Castillo, J., Noris, E., Moriones, E., Louro, D. (2000)
    Typing of tomato yellow leaf curl viruses in Europe
    in European journal of plant pathology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Accotto, G.P., Navas-Castillo, J., Noris, E., Moriones, E., Louro, D. (literal)
Pagina inizio
  • 179 (literal)
Pagina fine
  • 186 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://link.springer.com/content/pdf/10.1023%2FA%3A1008736023293.pdf (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 106 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 7 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 2 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • NCJ, ME CSIC, Estac Expt La Mayora, Malaga 29750, Spain LD, Direccao Geral Proteccao Culturas, P-2780 Oeiras, Portugal (literal)
Titolo
  • Typing of tomato yellow leaf curl viruses in Europe (literal)
Abstract
  • Tomato yellow leaf curl disease is spreading in southern Europe, where it has quickly become a serious problem. In recent years, several virus isolates have been characterised. Although with some genetic variability, all isolates found in Europe belong to one of two species Tomato yellow leaf curl-Sardinia (TYLCV-Sar) or Tomato yellow leaf curl-Israel (TYLCV-Is). Several methods were tested to identify and type TYLCV isolates from field samples: (1) RFLP of a DNA fragment amplified from the coat protein gene; (2) PAGE of a fragment amplified from the C2 gene; (3) dot-blot hybridisation. All methods enabled the detection of the TYLCVs and provided good indications for attributing them to one species or the other. However, for typing purposes, the RFLP method was the most reliable, due to the easily recognisable pattern produced by the two virus species present in Europe. Dot-blot hybridisation is less expensive for identifying TYLCVs in large numbers of samples, particularly when a mixture of two probes is used. PAGE of the C2 fragment is the fastest of the methods tested. (literal)
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