Single molecule fluorescence spectroscopy of pH sensitive oligonucleotide switches (Articolo in rivista)

Type
Label
  • Single molecule fluorescence spectroscopy of pH sensitive oligonucleotide switches (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Alternative label
  • Kolaric, B; Sliwa, M; Brucale, M; Vallee, RAL; Zuccheri, G; Samori, B; Hofkens, J; De Schryver, FC (2007)
    Single molecule fluorescence spectroscopy of pH sensitive oligonucleotide switches
    in Photochemical & photobiological sciences (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Kolaric, B; Sliwa, M; Brucale, M; Vallee, RAL; Zuccheri, G; Samori, B; Hofkens, J; De Schryver, FC (literal)
Pagina inizio
  • 614 (literal)
Pagina fine
  • 618 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 6 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Katholieke Univ Leuven, Inst nanoscale Phys & Chem, Dept Chem, B-3001 Heverlee, Belgium; Univ Bologna, Natl Inst Phys Matter, Dept Biochem G Moruzzi, I-40126 Bologna, Italy; Univ Bologna, Italian Interuniv Consortium Mat Sci & Technol, I-40126 Bologna, Italy (literal)
Titolo
  • Single molecule fluorescence spectroscopy of pH sensitive oligonucleotide switches (literal)
Abstract
  • Several authors demonstrated that an oligonucleotide based pH-sensitive construct can act as a switch between an open and a closed state by changing the pH. To validate this process, specially designed fluorescence dye-quencher substituted oligonucleotide constructs were developed to probe the switching between these two states. This paper reports on bulk and single molecule fluorescence investigations of a duplex-triplex pH sensitive oligonucleotide switch. On the bulk level, only a partial quenching of the fluorescence is observed, similarly to what is observed for other published switches and is supposed to be due to intermolecular interactions between oligonucleotide strands. On the single molecule level, each DNA-based nanometric construct shows a complete switching. These observations suggest the tendency of the DNA construct to associate at high concentration. (literal)
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