Characterization of biological features of a rat F98 GBM model: A PET-MRI study with [(18)F]FAZA and [(18)F]FDG (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Characterization of biological features of a rat F98 GBM model: A PET-MRI study with [(18)F]FAZA and [(18)F]FDG (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.nucmedbio.2013.05.004 (literal)

Alternative label

• Sara Belloli a, b, c, Andrea Brioschi d, Letterio Salvatore Politi e, Francesca Ronchetti d, Sara Calderoni d, Isabella Raccagni b, Antonella Pagani e, Cristina Monterisi a, b, c, Francesco Zenga f, g, Gianpaolo Zara h, Ferruccio Fazio a, b, c, Alessandro Mauro d, f, Rosa Maria Moresco a, b, c, (2013)
  Characterization of biological features of a rat F98 GBM model: A PET-MRI study with [(18)F]FAZA and [(18)F]FDG
  in Nuclear medicine and biology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Sara Belloli a, b, c, Andrea Brioschi d, Letterio Salvatore Politi e, Francesca Ronchetti d, Sara Calderoni d, Isabella Raccagni b, Antonella Pagani e, Cristina Monterisi a, b, c, Francesco Zenga f, g, Gianpaolo Zara h, Ferruccio Fazio a, b, c, Alessandro Mauro d, f, Rosa Maria Moresco a, b, c, (literal)

Pagina inizio

• 831 (literal)

Pagina fine

• 840 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 40 (literal)

Rivista

• Nuclear medicine and biology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 6 (literal)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• a. IBFM-CNR, Segrate, Italy. b. Fondazione Tecnomed and Department of Health Sciences, University of Milan-Bicocca, Milan, Italy. c. Department of Nuclear Medicine, San Raffaele Scientific Institute, Milan, Italy. d. Istituto Auxologico Italiano, Department of Neurology and Neurorehabilitation, San Giuseppe Hospital, Piancavallo (VB), Italy. e. Neuroradiology Department and Neuroradiology Research Group, San Raffaele Scientific Institute, Milan, Italy. f. Department of Neuroscience, University of Turin, Turin, Italy. g. City of Health and Science Hospital, Turin, Italy. h. Department of Science and Technology of Drug, University of Turin, Turin, Italy. (literal)

Titolo

• Characterization of biological features of a rat F98 GBM model: A PET-MRI study with [(18)F]FAZA and [(18)F]FDG (literal)

Abstract

• Introduction The prognosis of malignant gliomas remains largely unsatisfactory for the intrinsic characteristics of the pathology and for the delayed diagnosis. Multimodal imaging based on PET and MRI may assess the dynamics of disease onset and progression allowing the validation of preclinical models of glioblastoma multiforme (GBM). The aim of this study was the characterization of a syngeneic rat model of GBM using combined in vivo imaging and immunohistochemistry. Methods Four groups of Fischer rats were implanted in a subcortical region with increasing concentration of rat glioma F98 cells and weekly monitored with Gd-MR, [18F]FDG- and [18F]FAZA-PET starting one week after surgery. Different targets were evaluated on post mortem brain specimens using immunohistochemistry: VEGF, GFAP, HIF-1?, Ki-67 and nestin. Results Imaging results indicated that tumor onset but not progression was related to the number of F98 cells. Hypoxic regions identified with [18F]FAZA and high-glucose metabolism regions recognized with [18F]FDG were located respectively in the core and in external areas of the tumor, with partial overlap and remodeling during disease progression. Histological and immunohistochemical analysis confirmed PET/MRI results and revealed that our model resumes biological characteristics of human GBM. IHC and PET studies showed that necrotic regions, defined on the basis of [18F]FDG uptake reduction, may include hypoxic clusters of vital tumor tissue identified with [18F]FAZA. This last information is particularly relevant for the identification of the target volume during image-guided radiotherapy. Conclusions In conclusion, the combined use of PET and MRI allows in vivo monitoring of the biological modification of F98 lesions during tumor progression. (literal)

Prodotto di

• Institute of molecular bioimaging and physiology (IBFM) (Istituto)
• Imaging pre-clinico (ME.P06.006.002) (Modulo)

Autore CNR

• ROSA MARIA MORESCO (Persona)
• Cristina Monterisi (Unità di personale esterno)
• SARA BELLOLI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Characterization of biological features of a rat F98 GBM model: A PET-MRI study with [(18)F]FAZA and [(18)F]FDG" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ROSA MARIA MORESCO (Persona)
• SARA BELLOLI (Unità di personale interno)
• Cristina Monterisi (Unità di personale esterno)

Prodotto

• Institute of molecular bioimaging and physiology (IBFM) (Istituto)
• Imaging pre-clinico (ME.P06.006.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Nuclear medicine and biology (Rivista)

Insieme di parole chiave di

• Keywords of "Characterization of biological features of a rat F98 GBM model: A PET-MRI study with [(18)F]FAZA and [(18)F]FDG" (Insieme di parole chiave)