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Maturation Promoting Factor in ascidian oocytes is regulated by different intracellular signals at meiosis I and II (Articolo in rivista)

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Maturation Promoting Factor in ascidian oocytes is regulated by different intracellular signals at meiosis I and II (Articolo in rivista) (literal)

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Russo, G.L., Kyozuka, K., Antonazzo, L., Tosti, E. and Dale, B. (1996)
Maturation Promoting Factor in ascidian oocytes is regulated by different intracellular signals at meiosis I and II
in Development (Camb.); Company of biologists, Cambridge (Regno Unito)
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cited By (since 1996)55 Questa pubblicazione si riferisce a un periodo in cui l'autore non lavorava presso il CNR Free downloadable at: http://dev.biologists.org/content/122/7/1995.short (literal)

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Stazione Zoologica 'Anton Dohrn', Villa Comunale, 80121, Naples, Italy; Asamushi Marine Biological Station, Asamushi, Aomori 039-34, Japan (literal)

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Maturation Promoting Factor in ascidian oocytes is regulated by different intracellular signals at meiosis I and II (literal)

Abstract

Using the fluorescent dye Calcium Green-dextran, we measured intracellular Ca 2+ in oocytes of the ascidian Ciona intestinalis at fertilization and during progression through meiosis. The relative fluorescence intensity increased shortly after insemination in a single transient, the activation peak, and this was followed by several smaller oscillations that lasted for approximately 5 minutes (phase 1). The first polar body was extruded after the completion of the phase 1 transients, about 9 minutes after insemination, and then the intracellular calcium level remained at baseline for a period of 5 minutes (phase 2). At 14 minutes postinsemination a second series of oscillations was initiated that lasted 11 minutes (phase 3) and terminated at the time of second polar body extrusion. Phases 1 and 3 were inhibited by preloading oocytes with 5 mM heparin. Simultaneous measurements of membrane currents, in the whole-cell clamp configuration, showed that the 1-2 nA inward fertilization current correlated temporally with the activation peak, while a series of smaller oscillations of 0.1-0.3 nA amplitude were generated at the time of the phase 3 oscillations. (literal)

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