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ADP-ribose gates the fertilization channel in ascidian oocytes (Articolo in rivista)

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ADP-ribose gates the fertilization channel in ascidian oocytes (Articolo in rivista) (literal)

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Wilding, M. and Russo, G.L. and Galione, A. and Marino, M. and Dale, B. (1998)
ADP-ribose gates the fertilization channel in ascidian oocytes
in American journal of physiology. Cell physiology; American Physiological Society, Bethesda (Stati Uniti d'America)
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Wilding, M. and Russo, G.L. and Galione, A. and Marino, M. and Dale, B. (literal)

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Stazione Zoologica Anton Dohrn, 80121 Naples, Italy; Inst. of Food Science and Technology, 83100 Avellino, Italy; Univ. Department of Pharmacology, Mansfield Road, Oxford OX1 3QT, United Kingdom; Stazione Zoologica Anton Dohrn, Villa Comunale 1, 80121 Naples, Italy (literal)

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Abstract

We report an ion channel in the plasma membrane of unfertilized oocytes of the ascidian Ciona intestinalis that is directly gated by the second messenger ADP-ribose. The ion channel is permeable to Ca 2+ and Na + and is characterized by a reversal potential between 0 and +20 mV and a unitary conductance of 140 pS. Preinjection of the Ca 2+ chelator 1,2-bis(2- aminophenoxy)ethane,N,N,N',N'-tetraacetic acid (BAPTA) or antagonists of intracellular Ca 2+ release channels into oocytes did not inhibit the ADP- ribose current, demonstrating that the channel is activated in a Ca 2+- independent manner. Both the fertilization current and the current induced by the injection of nicotinamide nucleotides are blocked by nicotinamide, suggesting that the ADP-ribose channel is activated at fertilization in a nicotinamide-sensitive manner. These data suggest that ascidian sperm trigger the hydrolysis of nicotinamide nucleotides in the oocyte to ADP-ribose and that this mechanism is responsible for the production of the fertilization current. (literal)

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