GeneUp: A program to select short PCR primer pairs that occur in multiple members of sequence lists (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• GeneUp: A program to select short PCR primer pairs that occur in multiple members of sequence lists (Articolo in rivista) (literal)

Anno

• 1999-01-01T00:00:00+01:00 (literal)

Alternative label

• Pesole, G.; Liuni, S.; Grillo, G.; Belichard, P.; Trenkle, T.; Welsh, J.; McClelland, M. (1999)
  GeneUp: A program to select short PCR primer pairs that occur in multiple members of sequence lists
  in BioTechniques
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Pesole, G.; Liuni, S.; Grillo, G.; Belichard, P.; Trenkle, T.; Welsh, J.; McClelland, M. (literal)

Pagina inizio

• 112 (literal)

Pagina fine

• 116 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 25 (literal)

Rivista

• BioTechniques (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Universitá della Basilicata, Potenza, 1 Centro di Studio sui Mitocondri e Metabolismo Energetico,Bari, Universitá di Bari, Bari, Italy Sidney Kimmel Cancer Center, San Diego, CA, USA (literal)

Titolo

• GeneUp: A program to select short PCR primer pairs that occur in multiple members of sequence lists (literal)

Abstract

• A computer program is presented that selects a small set of short primer pairs for PCR to sample all the sequences in a user-specified list of mRNAs. Such primer pairs could be used to increase the probability of sampling mRNAs of particular interest in differential display and to generate simplified hybridization probes for DNA chips or arrays. The program uses simulated PCR to find pairs of primers that sample more than one sequence in the list. A small set of such primer pairs is selected that give maximal coverage of the sequences in the list. Primer pairs are excluded that: (i) generate simulated PCR products of the same size from a number sequences irt the list, (ii) can easily form primer dimers, (iii) are outside a specified range of G + C content or (iv) occur in another list of undesirable sequences, such as rRNAs and Alu repeats. Five lists consisting of from 48-285 cDNA sequences were used to test the program. A small number of pairs of primers, 8-10 bases in length, were selected that fit the above criteria and that generate one or more simulated PCR products in all or most of the cDNAs in each list. (literal)

Prodotto di

• Bioinformatica per la Genomica Funzionale e Comparata (SV.P18.004.001) (Modulo)

Autore CNR

• SABINO LIUNI (Unità di personale interno)
• GIORGIO GRILLO (Unità di personale interno)

Incoming links:

Autore CNR di

• GIORGIO GRILLO (Unità di personale interno)
• SABINO LIUNI (Unità di personale interno)

Prodotto

• Bioinformatica per la Genomica Funzionale e Comparata (SV.P18.004.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• BioTechniques (Rivista)