A comparison of molecular diagnostic procedures for the detection of aster yellows phytoplasmas (16Sr-I) in leafhopper vectors (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• A comparison of molecular diagnostic procedures for the detection of aster yellows phytoplasmas (16Sr-I) in leafhopper vectors (Articolo in rivista) (literal)

Anno

• 2003-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/BF02979779 (literal)

Alternative label

• Bertin, S., Palermo, S., Marzachi, C., Bosco, D. (2003)
  A comparison of molecular diagnostic procedures for the detection of aster yellows phytoplasmas (16Sr-I) in leafhopper vectors
  in Phytoparasitica
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Bertin, S., Palermo, S., Marzachi, C., Bosco, D. (literal)

Pagina inizio

• 141 (literal)

Pagina fine

• 145 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 32 (literal)

Rivista

• Phytoparasitica (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 5 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 2 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• BS, PS, BD: Istituto di Virologia Vegetale, CNR, Torino, Italia BS, PS, BD: Università di Torino, Italia (literal)

Titolo

• A comparison of molecular diagnostic procedures for the detection of aster yellows phytoplasmas (16Sr-I) in leafhopper vectors (literal)

Abstract

• Different molecular procedures were compared for the detection of aster yellows phytoplasmas (AYP) in the leafhopper vectors Macrosteles quadripunctulatus (Kirschbaum), Euscelidius variegatus (Kirschbaum) and Euscelis incisus (Kirschbaum). Polymerase chain reaction (PCR) with universal and group-specific primers designed on the 16S-rDNA sequence was most sensitive in nested assays. A dot-blot procedure with an oligoprobe designed on the 16S-rDNA was less sensitive and consistent to detect phytoplasmas in total insect DNA, but consistently detected amplicons from direct PCR. The dot-blot assay with a probe based on a phytoplasma plasmid sequence detected AYP in most vector specimens and did not react with DNAs from leafhoppers infected by flavescence doree and psyllids infected by apple proliferation phytoplasmas. This last assay is almost devoid of contamination risks, faster and cheaper compared to PCR, therefore it has to be preferred for field-scale analysis of leafhopper populations. (literal)

Prodotto di

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Autore CNR

• CRISTINA MARZACHI' (Persona)

Insieme di parole chiave

• Keywords of "A comparison of molecular diagnostic procedures for the detection of aster yellows phytoplasmas (16Sr-I) in leafhopper vectors" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• CRISTINA MARZACHI' (Persona)

Prodotto

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Phytoparasitica (Rivista)

Insieme di parole chiave di

• Keywords of "A comparison of molecular diagnostic procedures for the detection of aster yellows phytoplasmas (16Sr-I) in leafhopper vectors" (Insieme di parole chiave)