The Human Tim-Tipin Complex Interacts Directly with DNA Polymerase {epsilon} and Stimulates Its Synthetic Activity. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• The Human Tim-Tipin Complex Interacts Directly with DNA Polymerase {epsilon} and Stimulates Its Synthetic Activity. (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1042/BJ20130059 (literal)

Alternative label

• Aria V, De Felice M, Di Perna R, Uno S, Masai H, Syväoja JE, van Loon B, Hübscher U, Pisani FM (2013)
  The Human Tim-Tipin Complex Interacts Directly with DNA Polymerase {epsilon} and Stimulates Its Synthetic Activity.
  in Journal of biological chemistry (Online)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Aria V, De Felice M, Di Perna R, Uno S, Masai H, Syväoja JE, van Loon B, Hübscher U, Pisani FM (literal)

Pagina inizio

• 12742 (literal)

Pagina fine

• 12752 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 288 (literal)

Rivista

• Journal of biological chemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Biochimica delle Proteine - CNR, Napoli. Italia Tokyo Metropolitan Institute of Medical Science, Tokyo. Japan University of Eastern Finland, Kuopio. Finland University of Zurich-Irchel, Zurich. Switzerland. (literal)

Titolo

• The Human Tim-Tipin Complex Interacts Directly with DNA Polymerase {epsilon} and Stimulates Its Synthetic Activity. (literal)

Abstract

• The Tim-Tipin complex plays an important role in the S phase checkpoint and replication fork stability in metazoans, but the molecular mechanism underlying its biological function is poorly understood. Here, we present evidence that the recombinant human Tim-Tipin complex (and Tim alone) markedly enhances the synthetic activity of DNA polymerase ?. In contrast, no significant effect on the synthetic ability of human DNA polymerase ? and ? by Tim-Tipin was observed. Surface plasmon resonance measurements and co-immunoprecipitation experiments revealed that recombinant DNA polymerase ? directly interacts with either Tim or Tipin. In addition, the results of DNA band shift assays suggest that the Tim-Tipin complex (or Tim alone) is able to associate with DNA polymerase ? bound to a 40-/80-mer DNA ligand. Our results are discussed in view of the molecular dynamics at the human DNA replication fork. (literal)

Prodotto di

• Institute of protein biochemistry (IBP) (Istituto)
• DR.SSA PISANI: Studio dei fattori proteici coinvolti nella replicazione del DNA e nei meccanismi di checkpoint della fase S del ciclo cellulare (SV.P17.002.001) (Modulo)

Autore CNR

• Valentina Aria (Persona)
• MARIARITA DE FELICE (Persona)
• FRANCESCA MARIA PISANI (Persona)

Insieme di parole chiave

• Parole chiave di "The Human Tim-Tipin Complex Interacts Directly with DNA Polymerase {epsilon} and Stimulates Its Synthetic Activity." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• MARIARITA DE FELICE (Persona)
• FRANCESCA MARIA PISANI (Persona)
• Valentina Aria (Persona)

Prodotto

• Institute of protein biochemistry (IBP) (Istituto)
• DR.SSA PISANI: Studio dei fattori proteici coinvolti nella replicazione del DNA e nei meccanismi di checkpoint della fase S del ciclo cellulare (SV.P17.002.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of biological chemistry (Rivista)

Insieme di parole chiave di

• Parole chiave di "The Human Tim-Tipin Complex Interacts Directly with DNA Polymerase {epsilon} and Stimulates Its Synthetic Activity." (Insieme di parole chiave)