http://www.cnr.it/ontology/cnr/individuo/prodotto/ID243100

Construction of a pilot human YAC library in a recombination-defective yeast strain. (Articolo in rivista)

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Construction of a pilot human YAC library in a recombination-defective yeast strain. (Articolo in rivista) (literal)

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Palmieri G, Miano MG, Casamassimi A, Lania A, Kohno K, Schlessinger D, D'Urso M, Featherstone T. (1997)
Construction of a pilot human YAC library in a recombination-defective yeast strain.
in Gene (Amst.)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Palmieri G, Miano MG, Casamassimi A, Lania A, Kohno K, Schlessinger D, D'Urso M, Featherstone T. (literal)

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International Institute of Genetics and Biophysics, Naples 80125 Italy Kyoto Pharmaceutical University, Institute of Molecular and Cellular Biology, for Pharmaceutical Sciences, Kyoto 607 Japan Center for Genetics in Medicine, Department of Genetics, Washington University School of Medicine, St. Louis, MO USA (literal)

Titolo

Construction of a pilot human YAC library in a recombination-defective yeast strain. (literal)

Abstract

Using high-molecular-weight DNA fragments from a human lymphoblastoid cell line, a pilot collection of 2500 YACs was constructed in YKK115, a recombination-deficient strain of Saccharomyces cerevisiae carrying mutations in both the rad51 and rad52 genes. Analysis of 520 clones from the current library by pulsed-field gel electrophoresis revealed more than 97% single YACs with an insert size averaging 340 kb. Fluorescent in situ hybridization (FISH) performed with 37 clones on metaphase chromosomes suggested a high proportion mapping at centromeric (7) or telomeric (4) locations. The results are consistent with the stabilization of YACs in strains disarmed in recombination functions [Kohno, K., Oshiro, T., Kishine, H., Wada, M., Takeda, H., Ihara, N., Imamoto, F., Kano, Y. and Schlessinger, D. (1997) Human YACs unstable in a rad52 single mutant strain become stable in rad51rad52 double mutant. Gene, 000, 000-000 (GENE 10429)], and further suggest that the YACs may include regions that have been difficult to clone in other strains. (literal)

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