HPLC ANALYSIS OF CYCLIC ADENOSINE-DIPHOSPHATE RIBOSE AND ADENOSINE-DIPHOSPHATE RIBOSE - DETERMINATION OF NAD(+) METABOLITES IN HIPPOCAMPAL MEMBRANES (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• HPLC ANALYSIS OF CYCLIC ADENOSINE-DIPHOSPHATE RIBOSE AND ADENOSINE-DIPHOSPHATE RIBOSE - DETERMINATION OF NAD(+) METABOLITES IN HIPPOCAMPAL MEMBRANES (Articolo in rivista) (literal)

Anno

• 1995-01-01T00:00:00+01:00 (literal)

Alternative label

• Casabona G, Sturiale L, L'Episcopo MR, Raciti G, Fazzio A, Sarpietro MG, Genazzani AA, Cambria A, Nicoletti F (1995)
  HPLC ANALYSIS OF CYCLIC ADENOSINE-DIPHOSPHATE RIBOSE AND ADENOSINE-DIPHOSPHATE RIBOSE - DETERMINATION OF NAD(+) METABOLITES IN HIPPOCAMPAL MEMBRANES
  in Italian Journal of Biochemistry
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Casabona G, Sturiale L, L'Episcopo MR, Raciti G, Fazzio A, Sarpietro MG, Genazzani AA, Cambria A, Nicoletti F (literal)

Pagina inizio

• 258 (literal)

Pagina fine

• 268 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 44 (literal)

Rivista

• Italian Journal of Biochemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 11 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 5 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR, IST CHIM & TECNOL MAT POLIMERICI, CATANIA UNIV CATANIA,IST SCI BIOCHIM & FARMACOL, CATANIA UNIV PERUGIA, FAC FARM, DIPARTIMENTO MED SPERIMENTALE & SCI BIOCHIM, PERUGIA (literal)

Titolo

• HPLC ANALYSIS OF CYCLIC ADENOSINE-DIPHOSPHATE RIBOSE AND ADENOSINE-DIPHOSPHATE RIBOSE - DETERMINATION OF NAD(+) METABOLITES IN HIPPOCAMPAL MEMBRANES (literal)

Abstract

• Cyclic adenosine diphosphate-ribose (cADPR) and ADPR were separated by high-performance liquid chromatography (HPLC) on a CarboPac PA-1 column at strong basic pH and quantitated by a pulsed amperometric detector. Although this HPLC method was quite sensitive and highly reproducible, it did not allow the separation of cADPR from guanosine monophosphate (GMP) which, when present, could be removed by ion-affinity chromatography, using gel-immobilized Fe3+ columns. Crude synaptic membranes from rat hippocampi were incubated with nicotinamide adenine dinucleotide (NAD) and acidic extracts were subject to HPLC analysis after neutralization. Incubation led to a time-dependent formation of ADPR, which was amplified when membranes were incubated in the presence of guanosine trisphosphate (GTP), guanosine-5'-0-(3-thiotrisphosphate) (GTP-gamma-S) or AlF3. cADPR did not accumulate in detectable amounts and only a minimal proportion (< 5%) of radioactivity originating from [3H]NAD co-eluted with authentic cADPR in extracts from hippocampal membranes. The simultaneous detection of cADPR and ADPR we have described may help the search for inhibitors of cADPR metabolism, which will allow to measure the cADPR that accumulates under basal conditions or in response to extracellular signals. (literal)

Prodotto di

• Institute of chemistry and technology of polymers (ICTP) (Istituto)

Autore CNR

• LUISELLA STURIALE (Persona)

Insieme di parole chiave

• Parole chiave di "HPLC ANALYSIS OF CYCLIC ADENOSINE-DIPHOSPHATE RIBOSE AND ADENOSINE-DIPHOSPHATE RIBOSE - DETERMINATION OF NAD(+) METABOLITES IN HIPPOCAMPAL MEMBRANES" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of chemistry and technology of polymers (ICTP) (Istituto)

Autore CNR di

• LUISELLA STURIALE (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Italian Journal of Biochemistry (Rivista)

Insieme di parole chiave di

• Parole chiave di "HPLC ANALYSIS OF CYCLIC ADENOSINE-DIPHOSPHATE RIBOSE AND ADENOSINE-DIPHOSPHATE RIBOSE - DETERMINATION OF NAD(+) METABOLITES IN HIPPOCAMPAL MEMBRANES" (Insieme di parole chiave)