A proteomic approach to the investigation of early events involved in vascular smooth muscle cell activation (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• A proteomic approach to the investigation of early events involved in vascular smooth muscle cell activation (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Alternative label

• Boccardi C.; Cecchettini A.; Caselli A.; Camici G.; Evangelista M.; Mercatanti A.; Rainaldi G.; Citti L. (2007)
  A proteomic approach to the investigation of early events involved in vascular smooth muscle cell activation
  in Cell and tissue research (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Boccardi C.; Cecchettini A.; Caselli A.; Camici G.; Evangelista M.; Mercatanti A.; Rainaldi G.; Citti L. (literal)

Pagina inizio

• 185 (literal)

Pagina fine

• 195 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 328 (literal)

Rivista

• Cell and tissue research (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• In: Cell and Tissue Research, vol. 328 (1) pp. 185 - 195. Springer-Verlag, 2007. (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Department of Biochemical Sciences, University of Florence, Florence, Italy Department of Human Morphology and Applied Biology, University of Pisa, Pisa, Itay Laboratory of Molecular and Gene Therapy, Clinical Physiology Institute CNR, Pisa, Italy (literal)

Titolo

• A proteomic approach to the investigation of early events involved in vascular smooth muscle cell activation (literal)

Abstract

• Vascular smooth muscle cells (VSMC) are mature cells that maintain great plasticity. This distinctive feature is the basis of the VSMC migration and proliferation involved in cardiovascular diseases. We have used a proteomic approach to the molecular changes that promote the switch of VSMC from having a quiescent to activated-proliferating phenotype. In particular, we have focused on modulations occurring during tyrosine-phosphorylation following cell activation by serum or single growth factors, such as insulin-like growth factor 1 or platelet-derived growth factor. A comparison of two-dimensional polyacryl-amide gel profiles from quiescent or activated-proliferating VSMC has allowed us to recognize a number of differences in protein expression. Several differentially expressed proteins have been identified by mass spectrometry, and their time-course changes during tyrosine-phosphorylation have been documented from time zero till 48 h after stimulation. We have documented a general decrease of the tyrosine-phosphorylation level within the first few minutes after stimulation followed by a recovery that is quick and dramatic for some chaperones and redox enzymes but not so significant for enzymes of glucose metabolism. With regard to cytoskeleton components, no remarkable fluctuations have been detected at the earliest time points, except for those relative to alfa-actin, which displays an impressive decrease. A comparison of the early stages of cell stimulation after the administration of serum or single growth factors has brought to light important differences in the phosphorylation of chaperones, thereby suggesting their crucial role in VSMC activation. (literal)

Prodotto di

• Istituto di fisiologia clinica (IFC) (Istituto)
• Tassonomia clinica molecolare - Tassonomia clinica molecolare (ME.P05.003.001) (Modulo)

Autore CNR

• MONICA EVANGELISTA (Unità di personale interno)
• LORENZO CITTI (Persona)
• ALBERTO MERCATANTI (Persona)
• GIUSEPPE RAINALDI (Persona)
• CLAUDIA BOCCARDI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "A proteomic approach to the investigation of early events involved in vascular smooth muscle cell activation" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• CLAUDIA BOCCARDI (Unità di personale interno)
• GIUSEPPE RAINALDI (Persona)
• LORENZO CITTI (Persona)
• MONICA EVANGELISTA (Unità di personale interno)
• ALBERTO MERCATANTI (Persona)

Prodotto

• Istituto di fisiologia clinica (IFC) (Istituto)
• Tassonomia clinica molecolare - Tassonomia clinica molecolare (ME.P05.003.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Cell and tissue research (Rivista)

Insieme di parole chiave di

• Keywords of "A proteomic approach to the investigation of early events involved in vascular smooth muscle cell activation" (Insieme di parole chiave)