Identification of a high-molecular-weight cadmium-binding protein in copper-resistant Bacillus acidocaldarius cells (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Identification of a high-molecular-weight cadmium-binding protein in copper-resistant Bacillus acidocaldarius cells (Articolo in rivista) (literal)

Anno

• 1996-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/0923-2508(96)81389-1 (literal)

Alternative label

• C Capasso, F Nazzaro, F Marulli, A Capasso, F La Cara, E Parisi (1996)
  Identification of a high-molecular-weight cadmium-binding protein in copper-resistant Bacillus acidocaldarius cells
  in Research in microbiology (Paris)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• C Capasso, F Nazzaro, F Marulli, A Capasso, F La Cara, E Parisi (literal)

Pagina inizio

• 287 (literal)

Pagina fine

• 296 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 147 (literal)

Rivista

• Research in microbiology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 4 (literal)

Note

• ubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• F.Nazzaro:ISA-CNR C.Capasso, A.Capasso, F.La Cara: IBP-CNR (literal)

Titolo

• Identification of a high-molecular-weight cadmium-binding protein in copper-resistant Bacillus acidocaldarius cells (literal)

Abstract

• Bacillus acidocaldarius grown in the presence of Cu++ was capable of accumulating the metal in the form of a protease-sensitive high molecular weight (HMW) moiety whose formation was inhibited by actinomycin D. Only cells preadapted in Cu++ were able to grow in a Cd(++)-containing medium. A cell-free extract from cadmium-stressed cells was fractionated by gel-permeation chromatography. The majority of cadmium was found associated with a HMW protein fraction which was further purified by anion exchange chromatography and high-performance liquid chromatography. The molecular weight of the purified protein was estimated to be 23,000 by SDS-PAGE. Amino acid analysis showed a low cysteine content and an abundance of aspartate and glutamate. It is likely that the cadmium-binding protein is an essential component of the mechanism mediating recovery from heavy metal toxicity. (literal)

Prodotto di

• Institute of protein biochemistry (IBP) (Istituto)
• Institute of food sciences (ISA) (Istituto)
• Tecnologie, Tracciabilità e Sicurezza degli Alimenti (AG.P05.004.001) (Modulo)

Autore CNR

• CLEMENTE CAPASSO (Unità di personale interno)
• FILOMENA NAZZARO (Persona)
• ANTONIO CAPASSO (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Identification of a high-molecular-weight cadmium-binding protein in copper-resistant Bacillus acidocaldarius cells" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of food sciences (ISA) (Istituto)
• Institute of protein biochemistry (IBP) (Istituto)
• Tecnologie, Tracciabilità e Sicurezza degli Alimenti (AG.P05.004.001) (Modulo)

Autore CNR di

• FILOMENA NAZZARO (Persona)
• CLEMENTE CAPASSO (Unità di personale interno)
• ANTONIO CAPASSO (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Research in microbiology (Rivista)

Insieme di parole chiave di

• Parole chiave di "Identification of a high-molecular-weight cadmium-binding protein in copper-resistant Bacillus acidocaldarius cells" (Insieme di parole chiave)