Efficacy of amphypathic oligopeptide to shuttle and relase a cis-acting DNA decoy into human cells (Articolo in rivista)

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  • Efficacy of amphypathic oligopeptide to shuttle and relase a cis-acting DNA decoy into human cells (Articolo in rivista) (literal)
Anno
  • 2002-01-01T00:00:00+01:00 (literal)
Alternative label
  • Citti L., Rovero P., Colombo M.G., Mariani L., Poliseno L., Rainaldi G. (2002)
    Efficacy of amphypathic oligopeptide to shuttle and relase a cis-acting DNA decoy into human cells
    in BioTechniques
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Citti L., Rovero P., Colombo M.G., Mariani L., Poliseno L., Rainaldi G. (literal)
Pagina inizio
  • 172 (literal)
Pagina fine
  • 177 (literal)
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  • Rivista leader nel settore delle tecnologie biochimiche e molecolari con “impact factor” di 2,173 (anno 2002). (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 32 (literal)
Rivista
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  • Studio di veicoli oligopeptidici anfipatici come carriers per il trasferimento di corte sequenze di acido nucleico per l’induzione di inibizione genica (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Citti L., Colombo M.G., Mariani L., Poliseno L., Rainaldi G.: Istituto di Fisiologia Clinica del CNR di Pisa, Rovero P.: Dip. Scienze Farmaceutiche Università di Salerno (literal)
Titolo
  • Efficacy of amphypathic oligopeptide to shuttle and relase a cis-acting DNA decoy into human cells (literal)
Abstract
  • We investigated the ability of an amphipathic oligopeptide to carry a synthetic dsDNA oligonucleotide inside human cells. The oligonucleotide was designed as a decoy binding site for the transcriptional activator of the methylguanine-DNA methyltransferase (MGMT) gene. The complex oligopeptide and decoy were administered to MCF10A exponentially growing cells, and the uptake was monitored by flow cytometry. After a 1-h exposure, almost all of the MCF10A cells were fluorescent, indicating that all of the cells had been transfected. By increasing the time, the fluorescence intensity per cell rapidly increased to a plateau at the 8-h time point. RT-PCR analysis of the MGMT gene was used as the molecular readout of the intracellular activity of the DNA decoy. MCF10A cells transfected with the oligopeptide/decoy complex showed a strong reduction in MGMT mRNA. Here, we discuss the advantages of using amphipathic oligopeptides as carriers of short DNA sequences. (literal)
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