Analysis of commercial beverage products by size exclusion chromatography coupled with UV-vis absorbance detection and dynamic surface tension detection (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Analysis of commercial beverage products by size exclusion chromatography coupled with UV-vis absorbance detection and dynamic surface tension detection (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.talanta.2009.09.051 (literal)

Alternative label

• Pierce, Karisa M.; Bramanti, Emilia; Onor, Massimo; Spiniello, Roberto; Kangas, Alexandra; Skogerboe, Kristen J.; Synovec, Robert E. (2010)
  Analysis of commercial beverage products by size exclusion chromatography coupled with UV-vis absorbance detection and dynamic surface tension detection
  in Talanta (Oxf.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Pierce, Karisa M.; Bramanti, Emilia; Onor, Massimo; Spiniello, Roberto; Kangas, Alexandra; Skogerboe, Kristen J.; Synovec, Robert E. (literal)

Pagina inizio

• 1445 (literal)

Pagina fine

• 1451 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 80 (literal)

Rivista

• Talanta (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 7 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 3 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• [ 1 ] Seattle Pacific Univ, Dept Chem & Biochem, Seattle, WA 98119 USA [ 2 ] Ist & Proc Chim Fis, CNR, Natl Res Council, Lab Instrumental Analyt Chem, I-56124 Pisa, Italy [ 3 ] Seattle Univ, Dept Chem, Seattle, WA 98122 USA [ 4 ] Univ Washington, Ctr Proc Analyt Chem, Dept Chem, Seattle, WA 98195 USA (literal)

Titolo

• Analysis of commercial beverage products by size exclusion chromatography coupled with UV-vis absorbance detection and dynamic surface tension detection (literal)

Abstract

• Multidimensional analysis of instant coffee and barley beverage samples using size exclusion chromatography (SEC) combined with a dynamic surface tension detector (DSTD) and a UV-vis absorbance detector (UV) is reported. A unique finding of this study was the action of the tetrabutylammonium (TBA) cation as a modifying agent (with bromide as the counter anion) that substantially increased the surface pressure signal and sensitivity of many of the proteins in the chromatographically separated samples. The tetrabutylammonium bromide (TBAB) enhancement of the surface pressure signal was further investigated by studying the response of 12 commercial standard proteins (a-lactalbumin, beta-lactoglobulin, human serum albumin (HSA), albumin from chicken egg white (OVA), bovine serum albumin (BSA), hemoglobin, alpha-chymotrypsinogen A, cytochrome C, myoglobin, RNase A, carbonic anhydrase, and lysozyme) in buffer performed using flow injection analysis (FIA) coupled with the DSTD with and without various concentrations of TBAB. The FIA-DSTD data show that 1 mM TBAB enhances sensitivity of HSA detection, by lowering the limit of detection (LOD) from 2 mg/mL to 0.1 mg/mL Similarly, the LOD for BSA was reduced from 1 mg/mL to 0.2 mg/mL These FIA-DSTD experiments allowed the detection conditions to be optimized for further SEC-UV/DSTD experiments. Thus, the SEC-UV/DSTD system has been optimized and successfully applied to the selective analysis of surface-active protein fractions in a commercial instant coffee sample and in a soluble barley sample. The complementary selectivity of using the DSTD relative to an absorbance detector is also demonstrated. (C) 2009 Elsevier B.V. All rights reserved. (literal)

Prodotto di

• Chimica analitica strumentale (PM.P03.015.002) (Modulo)
• Istituto di chimica dei composti organo metallici (ICCOM) (Istituto)

Autore CNR

• MASSIMO ONOR (Persona)
• EMILIA BRAMANTI (Persona)
• ROBERTO SPINIELLO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Analysis of commercial beverage products by size exclusion chromatography coupled with UV-vis absorbance detection and dynamic surface tension detection" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di chimica dei composti organo metallici (ICCOM) (Istituto)
• Chimica analitica strumentale (PM.P03.015.002) (Modulo)

Autore CNR di

• MASSIMO ONOR (Persona)
• EMILIA BRAMANTI (Persona)
• ROBERTO SPINIELLO (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Talanta (Rivista)

Insieme di parole chiave di

• Keywords of "Analysis of commercial beverage products by size exclusion chromatography coupled with UV-vis absorbance detection and dynamic surface tension detection" (Insieme di parole chiave)