Insights into phytoplasma biology through second generation sequencing (Abstract/Comunicazione in atti di convegno)

Type
Label
  • Insights into phytoplasma biology through second generation sequencing (Abstract/Comunicazione in atti di convegno) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Alternative label
  • Palmano S., Saccardo F., Martini M., Ermacora P., Scortichini M., Abbà S., Marzachì C., Loi N., Firrao.G. (2012)
    Insights into phytoplasma biology through second generation sequencing
    in XVIII Convegno Nazionale Società Italiana di Patologia Vegetale, Sassari, Italia, 24-26 settembre 2012
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Palmano S., Saccardo F., Martini M., Ermacora P., Scortichini M., Abbà S., Marzachì C., Loi N., Firrao.G. (literal)
Pagina inizio
  • 41 (literal)
Note
  • Abstract (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • PA, AS, MC: Istituto di Virologia Vegetale, CNR, Torino, Italy SF,MM,EP, SM, LN, FG: Dipartimento di Scienze Agrarie ed Ambientali, Università di Udine, Udine, Italy (literal)
Titolo
  • Insights into phytoplasma biology through second generation sequencing (literal)
Abstract
  • In order to allow a comparative pathogenomics of phytoplasmas, we optimized a procedure which allowed the reconstruction of high quality genome drafts from Illumina reads, using standard DNA preparation from periwinkle. The genome analysis of four phytoplasma strains belonging to the 16SrII group (X disease clade), namely the Vaccinium Witches' Broom phytoplasma (647,754 nts in 272 contigs), the Italian Clover Phyllody phytoplasma strain MA (597245 nts in 197 contigs), the Poinsettia branch-inducing phytoplasma strain JR, (631440 nts in 185 contigs), and the Milkweed Yellows phytoplasma (583806 nts in 158 contigs) revealed similarities in their basic metabolic potential with the genomes of other wide host range phytoplasmas, and highlighted the role of membrane trafficking in defining virulence and host adaptation. The genome analysis was applied to compare two phytoplasmas related to \"Ca. Phytoplasma asteris\" and belonging to the 16SrI-B subgroup, namely the Chrysanthemum yellows phytoplasma (660013 nts in 253 contigs) and the Lettuce yellows phytoplasma (694813 nts in 205 contigs). Results obtained showed that, despite the strikingly different symptoms induced in the infected periwinkle, the two phytoplasmas share highly similar genomes. Moreover, the results of the genome sequencing of the six phytoplasmas pointed out the significant role of horizontal gene transfer among different \"Ca. Phytoplasma\" species in shaping phytoplasma genomes and promoting their diversity. (literal)
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