FRET-based protein-DNA binding assay for detection of active NF-kappa B (Articolo in rivista)

Type
Label
  • FRET-based protein-DNA binding assay for detection of active NF-kappa B (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.snb.2005.07.014 (literal)
Alternative label
  • Ambra Giannetti; Lorenzo Citti; Claudio Domenici; Lorena Tedeschi; Francesco Baldini; Musundi B. Wabuyele; Tuan Vo-Dinh (2006)
    FRET-based protein-DNA binding assay for detection of active NF-kappa B
    in Sensors and actuators. B, Chemical (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Ambra Giannetti; Lorenzo Citti; Claudio Domenici; Lorena Tedeschi; Francesco Baldini; Musundi B. Wabuyele; Tuan Vo-Dinh (literal)
Pagina inizio
  • 649 (literal)
Pagina fine
  • 654 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 113 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 2 (literal)
Note
  • Scopu (literal)
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Fisica Applicata CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy Istituto di Fisiologia Clinica, CNR, Via Moruzzi 1, 56124 Pisa, Italy Musundi B. Wabuyele, Tuan Vo-Dinh* Center for Advanced Biomedical Photonics Life Sciences Division, Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, TN, USA 37831 (literal)
Titolo
  • FRET-based protein-DNA binding assay for detection of active NF-kappa B (literal)
Abstract
  • The Nuclear Factor kappa B (NF-kB), a transcription factor regulating a battery of inflammatory genes, has been implicated as playing a fundamental role in the development of numerous pathological states. The NF-kB dimer is located in the cytoplasm of cells in an inactive form that can be activated by many stimuli (e.g. cytokines, bacterial or viral products, UV-radiation, etc.). It has been demonstrated that anti-inflammatory agents, both steroid and non steroid, besides their principal mechanism of action, have a secondary mechanism with anti-NF-kB effects. Therefore, when anti-inflammatory therapy is applied to an inflammatory disease, for which detection of the efficacy of the therapeutic agent is particularly important, monitoring of NF-kB-active form concentration (for example in cellular lysate) is an important target. The critical need for a simple and direct method to evaluate the presence of active NF-kB in a biological sample can be addressed using a suitable and reusable biosensor. For this purpose, a novel method, using fluorescence resonance energy transfer (FRET), to detect the active form of NF-kB binding a specific DNA sequence has been developed. In order to evaluate FRET due to the DNA/protein binding interaction taking place between dsDNA immobilized in a capillary wall and p50 proteins, a highly sensitive FRET-based biosensor system developed in our laboratory was used. (literal)
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