A comparative study of two isoforms of laccase secreted by the \"white-rot\" fungus Rigidoporus lignosus, exhibiting significant structural and functional differences (Articolo in rivista)

Type
Label
  • A comparative study of two isoforms of laccase secreted by the \"white-rot\" fungus Rigidoporus lignosus, exhibiting significant structural and functional differences (Articolo in rivista) (literal)
Anno
  • 1998-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/S0162-0134(98)10057-0 (literal)
Alternative label
  • R.P. Bonomo , A.M. Boudet , R. Cozzolino , E. Rizzarelli , A.M. Santoro , R. Sterjiades , R. Zappal?a (1998)
    A comparative study of two isoforms of laccase secreted by the "white-rot" fungus Rigidoporus lignosus, exhibiting significant structural and functional differences
    in Journal of inorganic biochemistry
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • R.P. Bonomo , A.M. Boudet , R. Cozzolino , E. Rizzarelli , A.M. Santoro , R. Sterjiades , R. Zappal?a (literal)
Pagina inizio
  • 205 (literal)
Pagina fine
  • 211 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 71 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Dipartimento di Scienze Chimiche, Universit?a di Catania, Viale A. Doria 6, 95125 Catania, Italy UMR CNRS/UPS5546 Centre de Biologie et Physiologie V?eg?etales, Universite Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, France Istituto per lo Studio delle Sostanze Naturali di Interesse Alimentare e Chimico-Farmaceutico, CNR, Sezione per lo studio di Modelli di Metallo-Enzimi, Viale A. Doria 6, 95125 Catania, Italy (literal)
Titolo
  • A comparative study of two isoforms of laccase secreted by the \"white-rot\" fungus Rigidoporus lignosus, exhibiting significant structural and functional differences (literal)
Abstract
  • Two isoforms of laccase were obtained as the predominant phenol-oxidases in de®ned medium liquid cultures of the ``white-rot'' fungus Rigidoporus lignosus (R. lignosus). A characterization of the two laccases was made in terms of molecular mass, isoelectric point, metal content and N-terminal sequence. Furthermore, in order to gain information on the structural features related to the metal centers, a study of their geometric arrangement and their redox ability was made. It turned out that the two isoenzymes greatly di€ered with regard to pH stability, catalytic and copper centers features. It is proposed that all such di€erences are dependent on the amino acid sequences, which cause a distortion of the copper sites, thus accounting for the redox potential values and kinetic properties. (literal)
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