Functional epitope mapping oh humen interleukin-1 beta by surface plasmon resonance (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Functional epitope mapping oh humen interleukin-1 beta by surface plasmon resonance (Articolo in rivista) (literal)

Anno

• 1997-01-01T00:00:00+01:00 (literal)

Alternative label

• D'Ettorre, C., De Chiara, G., Casadei, R., Boraschi, D., Tagliabue, A. (1997)
  Functional epitope mapping oh humen interleukin-1 beta by surface plasmon resonance
  in European cytokine network
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• D'Ettorre, C., De Chiara, G., Casadei, R., Boraschi, D., Tagliabue, A. (literal)

Pagina inizio

• 161 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 8 (literal)

Rivista

• European cytokine network (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 171 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 2 (literal)

Note

• Scopus (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Dompé Research Center, L'Aquila, Italy. (literal)

Titolo

• Functional epitope mapping oh humen interleukin-1 beta by surface plasmon resonance (literal)

Abstract

• A panel of monoclonal antibodies to human IL-1 beta has been used to probe its conformational and functional characteristics. Real time antibody-protein interaction was assessed by surface plasmon resonance with a BIAcore apparatus, in order to determine the kinetic and thermodynamic parameters of the interaction and to map the recognition sites of the antibodies on the IL-1 beta surface. Topological analysis was thus compared to the inhibitory capacity of antibodies for IL-1 beta bioactivity and binding to the activating receptor IL-1RI. This functional mapping analysis allows the following hypothesis. At least two discrete areas of IL-1 beta, located within the sequences 133-147 and 177-186 (as defined by mAbs MhC1 and BRhD2, respectively), are apparently involved in IL-1RI-independent agonist activity, and thus possibly take part in the interaction with the receptor accessory protein IL-1RAcP. Another area in the 133-147 sequence (defined by mAb BRhC3) is involved in agonist binding to its receptor CDw121a (IL-1RI), whereas a site recognized by mAb BRhG5 within the sequence 218-243 is selectively responsible for non-agonist binding to the activating receptor. The loop between the 4th and the 5th beta-strand, at the open end of the IL-1 beta-barrel structure, may possibly take part in both non-agonist binding to IL-1RI and in the interaction with IL-1RAcP. (literal)

Prodotto di

• Ricerca traslazionale ed outcome nelle patologie infiammatorie e degenerative (ME.P04.009.001) (Modulo)

Autore CNR

• GIOVANNA DE CHIARA (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Functional epitope mapping oh humen interleukin-1 beta by surface plasmon resonance" (Insieme di parole chiave)

Incoming links:

Prodotto

• Ricerca traslazionale ed outcome nelle patologie infiammatorie e degenerative (ME.P04.009.001) (Modulo)

Autore CNR di

• GIOVANNA DE CHIARA (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• European cytokine network (Rivista)

Insieme di parole chiave di

• Parole chiave di "Functional epitope mapping oh humen interleukin-1 beta by surface plasmon resonance" (Insieme di parole chiave)