A nitric oxide-dependent cross-talk between class I and III histone deacetylases accelerates skin repair (Articolo in rivista)

Type
Label
  • A nitric oxide-dependent cross-talk between class I and III histone deacetylases accelerates skin repair (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1074/jbc.M112.441816 (literal)
Alternative label
  • Spallotta Francesco1; Cencioni Chiara1; Straino Stefania2; Nanni Simona3; Rosati Jessica4; Artuso Simona5; Manni Isabella5; Colussi Claudia3;Piaggio Giulia5;Martelli Fabio 6; Valente Sergio7; Mai Antonello7; Capogrossi Maurizio C.2; Farsetti Antonella8; Gaetano Carlo9 (2013)
    A nitric oxide-dependent cross-talk between class I and III histone deacetylases accelerates skin repair
    in Journal of biological chemistry (Online)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Spallotta Francesco1; Cencioni Chiara1; Straino Stefania2; Nanni Simona3; Rosati Jessica4; Artuso Simona5; Manni Isabella5; Colussi Claudia3;Piaggio Giulia5;Martelli Fabio 6; Valente Sergio7; Mai Antonello7; Capogrossi Maurizio C.2; Farsetti Antonella8; Gaetano Carlo9 (literal)
Pagina inizio
  • 11004 (literal)
Pagina fine
  • 11012 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 288 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 16 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 Centro Cardiologico Monzino, Italy; 2 Istituto Dermopatico dell'Immacolata, Italy; 3 Universita Cattolica del Sacro Cuore, Italy; 4 CSS-Mendel, Rome, Italy; 5 Istituto Nazionale Tumori Regina Elena, Italy; 6 Policlinico San Donato, Italy; 7 Universita Sapienza, Italy; 8 Consiglio Nazionale delle ricerche (CNR), Italy; 9 Goethe University, Germany (literal)
Titolo
  • A nitric oxide-dependent cross-talk between class I and III histone deacetylases accelerates skin repair (literal)
Abstract
  • In a mouse model of skin repair we found that the class I-IIa histone deacetylase inhibitor trichostatin A accelerated tissue regeneration. Unexpectedly, this effect was suppressed by Sirtinol, a class III histone deacetylase (HDAC) (sirtuin)-selective inhibitor. The role of sirtuins (SIRTs) was then investigated by using resveratrol and a novel SIRT1-2-3 activator, the MC2562 compound we synthesized recently. Both resveratrol and MC2562 were effective in accelerating wound repair. The local administration of natural or synthetic SIRT activators, in fact, significantly accelerated skin regeneration by increasing keratinocyte proliferation. In vitro experiments revealed that the activation of SIRTs stimulated keratinocyte proliferation via endothelial NO synthase phosphorylation and NO production. In this condition, the class I member HDAC2 was found S-nitrosylated on cysteine, a post-transduction modification associated with loss of activity and DNA binding capacity. After deacetylase inhibitor or SIRT activator treatment, ChIP showed, in fact, a significant HDAC2 detachment from the promoter region of insulin growth factor I (IGF-I), fibroblast growth factor 10 (FGF-10), and Epithelial Growth Factor (EGF), which may be the final recipients and effectors of the SIRT-NO-HDAC signaling cascade. Consistently, the effect of SIRT activators was reduced in the presence of NG-nitro-L-arginine methyl ester (L-NAME), a general inhibitor of NO synthesis. In conclusion, the NO-dependent cross-talk among class III and I histone deacetylases suggests an unprecedented signaling pathway important for skin repair. (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Autore CNR di
Prodotto
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
Insieme di parole chiave di
data.CNR.it