An expression system for the single-step production of recombinant human amidated calcitonin (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• An expression system for the single-step production of recombinant human amidated calcitonin (Articolo in rivista) (literal)

Anno

• 1996-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1006/prep.1996.0052 (literal)

Alternative label

• Merli S, De Falco S, Verdoliva A, Tortora M, Villain M, Silvi P, Cassani G, Fassina G (1996)
  An expression system for the single-step production of recombinant human amidated calcitonin
  in Protein expression and purification (Print); Academic Press, San Diego (Stati Uniti d'America)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Merli S, De Falco S, Verdoliva A, Tortora M, Villain M, Silvi P, Cassani G, Fassina G (literal)

Pagina inizio

• 347 (literal)

Pagina fine

• 354 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 7 (literal)

Rivista

• Protein expression and purification (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 4 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• TECNOGEN SCPA,PROT ENGN UNIT AND MOLEC BIOL UNIT, I-81015 PIANA MONTE VERNA,CE,ITALY (literal)

Titolo

• An expression system for the single-step production of recombinant human amidated calcitonin (literal)

Abstract

• Amidating mouse pituitary cells (AtT-20) have been engineered to secrete human calcitonin (hCT) in the fully active amidated form, without the need of additional enzymatic or chemical modifications. The 141-residue human calcitonin precursor has first been cloned in the eucaryotic expression vector pRc/RSV, and the resulting plasmid pRc/RSV/hCT introduced in AtT-20 cells. After transfection, 122 independent clones resistant to G-418 were selected and screened for calcitonin production using a competitive ELISA specifically designed to detect the amidated form of calcitonin. One of these clones was amplified and showed expression of 17 ng/ml of hCT, with a 70% increase in productivity after cAMP treatment. Calcitonin was partially purified from culture medium by two sequential steps of reverse-phase chromatography and characterized in terms of immunoreactivity and molecular weight by TOF-MALDI mass spectroscopy, which confirmed the intended chemical nature and the presence of the C-terminal amidated residue. (literal)

Editore

• Academic Press (Editore)

Prodotto di

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Identificazione di regolatori del differenziamento, della motilità e dell'apoptosi delle cellule staminali (SV.P15.008.001) (Modulo)

Autore CNR

• SANDRO DE FALCO (Unità di personale interno)

Incoming links:

Prodotto

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Identificazione di regolatori del differenziamento, della motilità e dell'apoptosi delle cellule staminali (SV.P15.008.001) (Modulo)

Autore CNR di

• SANDRO DE FALCO (Unità di personale interno)

Editore di

• Academic Press (Editore)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Protein expression and purification (Rivista)