Amino acid substitution in par j 2 recombinant allergen and its effect on IgE binding capacity (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Amino acid substitution in par j 2 recombinant allergen and its effect on IgE binding capacity (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.3814/2009/343405 (literal)

Alternative label

• Domenico Nuzzo, Federica Pizzo, Giuseppe Albeggiani, Serafina Sciarrino, and Giovanni Duro (2009)
  Amino acid substitution in par j 2 recombinant allergen and its effect on IgE binding capacity
  in Scholarly Research Exchange (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Domenico Nuzzo, Federica Pizzo, Giuseppe Albeggiani, Serafina Sciarrino, and Giovanni Duro (literal)

Pagina inizio

• 1 (literal)

Pagina fine

• 5 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 2009 (literal)

Rivista

• Scholarly Research Exchange (Print) (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 5 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Instituto di Biomedicina e Immunologia Molecolare (IBIM), Consiglio Nazionale delle Ricerche (CNR), 90146 Palermo, Italy (literal)

Titolo

• Amino acid substitution in par j 2 recombinant allergen and its effect on IgE binding capacity (literal)

Abstract

• Therapeutic attempts to cure allergic diseases reduce symptoms without circumventing the onset of the allergic reaction. Specific immunotherapy (SIT), is the most commonly used treatment. Nevertheless, SIT may account for various adverse events. Therefore, different therapies have been developed in order to treat and prevent allergic reactions. Among these therapies, there is an increased interest in studying recombinant peptides mutated in the IgE binding site. Several studies have shown two major allergens of Parietaria judaica (Pj) named Par j 1 and Par j 2, which have been cloned and characterized by us. In our study we have fragmented the Par j 2 protein in order to determine the major epitopes recognized by human IgE and we used site-directed mutagenesis to identify potential amino acid residues involved in IgE binding. The IgE binding activity of the recombinant peptides was tested and the results showed that site-specific mutagenesis at positions K41, T42, T43, and C52 caused a loss of IgE binding. The goal of this work is to synthesize molecules which can induce a protective immune response against Pj. These molecules will be used in immunotherapy in order to create new vaccines for the treatment of Parietaria pollen allergy. (literal)

Prodotto di

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)
• Studio enzimatico e genetico di malattie metaboliche. (ME.P01.014.008) (Modulo)

Autore CNR

• GIOVANNI DURO (Persona)
• FEDERICA PIZZO (Unità di personale esterno)
• SERAFINA SCIARRINO (Persona)
• DOMENICO NUZZO (Persona)
• GIUSEPPE ALBEGGIANI (Unità di personale interno)

Incoming links:

Autore CNR di

• SERAFINA SCIARRINO (Persona)
• GIOVANNI DURO (Persona)
• GIUSEPPE ALBEGGIANI (Unità di personale interno)
• DOMENICO NUZZO (Persona)
• FEDERICA PIZZO (Unità di personale esterno)

Prodotto

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)
• Studio enzimatico e genetico di malattie metaboliche. (ME.P01.014.008) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Scholarly Research Exchange (Print) (Rivista)